Portal de Periódicos da CAPES

Alterations in sperm protein phosphorylation in male infertility

2001; Wiley; Volume: 33; Issue: 5 Linguagem: Inglês

10.1111/j.1439-0272.2001.tb01497.x

1439-0272

María Luisa Hortas, José Antonio Castilla, Moran Gil, Francisco Samaniego, M Morell, Maximino Redondo,

Ovarian function and disorders

AndrologiaVolume 33, Issue 5 p. 282-286 Alterations in sperm protein phosphorylation in male infertility M. L. Hortas, Corresponding Author M. L. Hortas Area de Biotecnologia, Hospital Costa del Sol, Marbella, SpainArea de Biotecnologia, Hospital 'Costa del Sol', Carretera de Cádiz, km 187, 29600 Marbella, Málaga, Spain. E-mail: mlhortas@hcs.esSearch for more papers by this authorJ. A. Castilla, J. A. Castilla Unidad de Reproducción, Hospital Virgen de las Nieves, Granada, SpainSearch for more papers by this authorM. T. Gil, M. T. Gil Servicio de Análisis Clinicos, Hospital Na Sra. del Prado, Talavera de la Reina, SpainSearch for more papers by this authorF. Samaniego, F. Samaniego Unidad de Reproducción, Hospital Virgen de las Nieves, Granada, SpainSearch for more papers by this authorM. Morell, M. Morell Dpto. Bioquimica y Biologia Molecular, Universidad de Málaga, Málaga, SpainSearch for more papers by this authorM. Redondo, M. Redondo Area de Biotecnologia, Hospital Costa del Sol, Marbella, SpainSearch for more papers by this author M. L. Hortas, Corresponding Author M. L. Hortas Area de Biotecnologia, Hospital Costa del Sol, Marbella, SpainArea de Biotecnologia, Hospital 'Costa del Sol', Carretera de Cádiz, km 187, 29600 Marbella, Málaga, Spain. E-mail: mlhortas@hcs.esSearch for more papers by this authorJ. A. Castilla, J. A. Castilla Unidad de Reproducción, Hospital Virgen de las Nieves, Granada, SpainSearch for more papers by this authorM. T. Gil, M. T. Gil Servicio de Análisis Clinicos, Hospital Na Sra. del Prado, Talavera de la Reina, SpainSearch for more papers by this authorF. Samaniego, F. Samaniego Unidad de Reproducción, Hospital Virgen de las Nieves, Granada, SpainSearch for more papers by this authorM. Morell, M. Morell Dpto. Bioquimica y Biologia Molecular, Universidad de Málaga, Málaga, SpainSearch for more papers by this authorM. Redondo, M. Redondo Area de Biotecnologia, Hospital Costa del Sol, Marbella, SpainSearch for more papers by this author First published: 24 April 2009 https://doi.org/10.1111/j.1439-0272.2001.tb01497.xCitations: 1AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Summary Protein phosphorylation is involved in sperm capacitation, so the effect of protein phosphatase inhibitors on the capacitation of spermatozoa of males with unexplained infertility was investigated. D-mannose ligand specific receptor expression in fresh, living spermatozoa, capacitated or treated with calyculin A (an inhibitor of protein phosphatases 1 and 2A), was studied in three groups of men: pre-vasectomy (fertile) males, males in couples with male infertility, and males in couples with infertility of unknown aetiology. Flow cytometry showed significant differences between infertile couples with a male factor and fertile couples (P<0.05), both after capacitation and after treatment with calyculin A. In the group of couples with infertility of unknown aetiology (n=15), D-mannose receptor expression was diminished in six cases after classical capacitation. However, when the spermatozoa of these six men were treated with calyculin A, five showed an increased specific D-mannose receptor expression. From these results it is suggested that in vitro treatment of spermatozoa with inhibitors of protein phosphatases may be of great value in some cases of unexplained infertility. Citing Literature Volume33, Issue5September 2001Pages 282-286 RelatedInformation