Produção Nacional
Revisado por pares
Immunology
2003; UNIVERSIDADE DE SÃO PAULO; Volume: 45; Issue: suppl 13 Linguagem: Inglês
10.1590/s0036-46652003000700009
ISSN1678-9946
AutoresPirmez, Oliveira-Neto, Oliveira, Ana F. Nogueira, Bianca Olivieri, H Modesto, Srini V. Kaveri, Vivian Ferreira do Amaral, Vasconcellos, Lorena Camargo Carneiro, K. F Tabosa, Maria CO Brígido, Marliane Batista Campos, R. Lainson, Fernando Tobias Sílveira, Campos De Carvalho, Cristiano Devenci Vendrame, Hiro Goto, Roberto Lemos, Joaquim Jorge Gomes Peixoto, Letícia Lintomen, José O. Previato, Lúcia Mendonça‐Previato, Gaspar Elsas, Maria Ignez C Gaspar Elsas, Maria Bellio, D. A. de Almeida, Hamílton Moreira,
Tópico(s)Immune Cell Function and Interaction
ResumoIn localized cutaneous leishmaniasis (LCL), the lesions are described as a chronic granulomatous inflammatory reaction composed of macrophages, T lymphocytes, plasma cells and mast cells.Despite of the T cell and type 1 cytokines are essential for the infection control, other cell types and inflammatory mediators may be important in this process.The aim of this study is to evaluate the expression of inflammatory mediators and mast cells subpopulations in human LCL lesions.The inflammatory infiltrate of LCL lesions caused by Leishmania (Viannia) braziliensis was analyzed by immunostaining using monoclonal antibodies that recognize inflammatory mediators (histamine, leukotriene B4/ LTB4 and prostaglandin F2a/PGF2a), mast cell proteases (tryptase and chymase) and iNOs.A total of 16 frozen biopsies obtained from localized cutaneous lesions were studied.The patients, all of them living in endemic areas of Rio de Janeiro, were classified in two groups: 9 with a period of evolution up to three months (early lesions) and 7 patients with more than three months of illness duration (late lesions).The number of cells expressing histamine was significantly elevated in early lesions, when compared to those with more chronic illness duration.Expression of PGF2a was found in both groups, with a slightly elevated amount of positive cells in early lesions.However, the number of LTB4 positive cells tended to increase in late lesions.These results indicate that histamine and lipid mediators could be involved not only in the initial stages of the inflammatory infiltrate development, but also in mechanisms that contribute to the progression of lesions.Both clinical groups presented similar patterns of iNOs expression.In patients with early lesions, the amount of tryptase+ cells was significantly higher than the chymase+ cells.However, in late lesions chymase+ cells were found in greater quantity.These results suggest that changes in mast cells phenotype could be involved in the immunopathogenesis of cutaneous lesions.
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