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Cloning of a Clostridium thermocellum DNA fragment encoding polypeptides that bind the catalytic components of the cellulosome

1992; Oxford University Press; Volume: 94; Issue: 1-2 Linguagem: Inglês

10.1111/j.1574-6968.1992.tb05308.x

1574-6968

Tsuchiyoshi Fujino, Pierre Béguin, Jean-Paul Aubert,

Bacteriophages and microbial interactions

A test based on the binding of ~-'51-1abeiled endoglucanase CelD was used to clone a DNA region encoding at least two different polypeptides that interact with the conserved reiterated segment present in many catalytic components of the Clostridium thermoceilum cellulosome.One of the polypeptides corresponds to the COOHterminal region of the S L (or SI) component of the cellulosome (U.T, Gerngross and A.L Demain, personal communication).It comprises repeated domains that are responsible for binding Iz~I-labelled CeiD, and presumably represent anchoring sites for the various catalytic components of the cellulosome.The other polypeptide is encoded by a gene that has not yet been described.