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EFFECTS OF MERCURY AND SELENIUM ON GLUTATHIONE METABOLISM AND OXIDATIVE STRESS IN MALLARD DUCKS

1998; Wiley; Volume: 17; Issue: 2 Linguagem: Inglês

10.1897/1551-5028(1998)017 2.3.co;2

1552-8618

David J. Hoffman, Gary H. Heinz,

Selenium in Biological Systems

Environmental Toxicology and ChemistryVolume 17, Issue 2 p. 161-166 Article Effects of mercury and selenium on glutathione metabolism and oxidative stress in mallard ducks† David J. Hoffman, Corresponding Author David J. Hoffman [email protected] Patuxent Wildlife Research Center, 12011 Beech Forest Drive, Laurel, Maryland 20708–4017, USAPatuxent Wildlife Research Center, 12011 Beech Forest Drive, Laurel, Maryland 20708–4017, USASearch for more papers by this authorGary H. Heinz, Gary H. Heinz Patuxent Wildlife Research Center, 12011 Beech Forest Drive, Laurel, Maryland 20708–4017, USASearch for more papers by this author David J. Hoffman, Corresponding Author David J. Hoffman [email protected] Patuxent Wildlife Research Center, 12011 Beech Forest Drive, Laurel, Maryland 20708–4017, USAPatuxent Wildlife Research Center, 12011 Beech Forest Drive, Laurel, Maryland 20708–4017, USASearch for more papers by this authorGary H. Heinz, Gary H. Heinz Patuxent Wildlife Research Center, 12011 Beech Forest Drive, Laurel, Maryland 20708–4017, USASearch for more papers by this author First published: 26 October 2009 https://doi.org/10.1002/etc.5620170204Citations: 103 † Presented at the Wildlife Mercury Conference, Fairfax, Virginia, USA, April 12–13, 1996. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Earlier studies have reported on the toxicity and related oxidative stress of different forms of Se, including seleno- D,L-methionine, in mallards (Anas platyrhynchos). This study compares the effects of Se (seleno-D,L-methionine) and Hg (methylmercury chloride) separately and in combination. Mallard drakes received one of the following diets: untreated feed (controls), or feed containing 10 ppm Se, 10 ppm Hg, or 10 ppm Se in combination with 10 ppm Hg. After 10 weeks, blood, liver, and brain samples were collected for biochemical assays. The following clinical and biochemical alterations occurred in response to Hg exposure: hematocrit and hemoglobin concentrations decreased; activities of the enzymes glutathione (GSH) peroxidase (plasma and liver), glutathione-S-transferase (liver), and glucose-6-phosphate dehydrogenase (G-6-PDH) (liver and brain) decreased; hepatic oxidized glutathione (GSSG) concentration increased relative to reduced glutathione (GSH); and lipid peroxidation in the brain was detected by increased thiobarbituric reactive substances (TBARS). Effects of Se alone included increased hepatic GSSG reductase activity and brain TBARS concentration. Selenium in combination with Hg partially or totally alleviated effects of Hg on GSH peroxidase, G-6-PDH, and GSSG. These findings are compared in relation to field observations for diving ducks and other aquatic birds. It is concluded that since both Hg and excess Se can affect thiol status, measurement of associated enzymes in conjunction with thiol status may be a useful bioindicator to discriminate between Hg and Se effects. The ability of Se to restore the activities of G-6-PDH, GSH peroxidase, and glutathione status involved in antioxidative defense mechanisms may be crucial to biological protection from the toxic effects of methylmercury. REFERENCES 1 Ohlendorf HM, Marois KC, Lowe RW, Harvey TE, Kelly PR. 1989. Environmental contaminants and diving ducks in San Francisco Bay. In Howard AD, ed, Selenium and Agricultural Drainage: Implications for San Francisco Bay and the California Environment. Proceedings, Fourth Selenium Symposium, Bay Institute of San Francisco, Sausalito, CA, USA, pp 60–69. 2 Ohlendorf HM, Hothem RL. 1995. Agricultural drainwater effects on wildlife in central California. In DJ Hoffman, DA Rattner, GA Burton, J Cairns, eds, Handbook of Ecotoxicology. 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