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A KRAB zinc finger protein gene is the potential target of 19q13 translocation in benign thyroid tumors

1999; Wiley; Volume: 26; Issue: 3 Linguagem: Inglês

10.1002/(sici)1098-2264(199911)26

1098-2264

Volkhard Rippe, Gazanfer Belge, Maren Meiboom, Bernd Kazmierczak, Alfredo Fusco, Jörn Bullerdiek,

Ubiquitin and proteasome pathways

Genes, Chromosomes and CancerVolume 26, Issue 3 p. 229-236 Research Article A KRAB zinc finger protein gene is the potential target of 19q13 translocation in benign thyroid tumors† Volkhard Rippe, Volkhard Rippe Center for Human Genetics and Genetic Counseling, University of Bremen, Bremen, GermanySearch for more papers by this authorGazanfer Belge, Gazanfer Belge Center for Human Genetics and Genetic Counseling, University of Bremen, Bremen, GermanySearch for more papers by this authorMaren Meiboom, Maren Meiboom Center for Human Genetics and Genetic Counseling, University of Bremen, Bremen, GermanySearch for more papers by this authorBernd Kazmierczak, Bernd Kazmierczak Center for Human Genetics and Genetic Counseling, University of Bremen, Bremen, GermanySearch for more papers by this authorAlfredo Fusco, Alfredo Fusco Dipartimento di Biologia e Patologia Cellulare e Molecolare, Faculta di Medicina e Chirurgia di Napoli, Naples, ItalySearch for more papers by this authorJörn Bullerdiek, Corresponding Author Jörn Bullerdiek [email protected] Center for Human Genetics and Genetic Counseling, University of Bremen, Bremen, GermanyCenter for Human Genetics and Genetic Counseling, University of Bremen, Leobenerstr. ZHG, D-28359 Bremen, Germany.Search for more papers by this author Volkhard Rippe, Volkhard Rippe Center for Human Genetics and Genetic Counseling, University of Bremen, Bremen, GermanySearch for more papers by this authorGazanfer Belge, Gazanfer Belge Center for Human Genetics and Genetic Counseling, University of Bremen, Bremen, GermanySearch for more papers by this authorMaren Meiboom, Maren Meiboom Center for Human Genetics and Genetic Counseling, University of Bremen, Bremen, GermanySearch for more papers by this authorBernd Kazmierczak, Bernd Kazmierczak Center for Human Genetics and Genetic Counseling, University of Bremen, Bremen, GermanySearch for more papers by this authorAlfredo Fusco, Alfredo Fusco Dipartimento di Biologia e Patologia Cellulare e Molecolare, Faculta di Medicina e Chirurgia di Napoli, Naples, ItalySearch for more papers by this authorJörn Bullerdiek, Corresponding Author Jörn Bullerdiek [email protected] Center for Human Genetics and Genetic Counseling, University of Bremen, Bremen, GermanyCenter for Human Genetics and Genetic Counseling, University of Bremen, Leobenerstr. ZHG, D-28359 Bremen, Germany.Search for more papers by this author First published: 28 September 1999 https://doi.org/10.1002/(SICI)1098-2264(199911)26:3 3.0.CO;2-JCitations: 11 † V.R. and G.B. contributed equally to this study. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract In an attempt to identify the target gene of specific translocations involving chromosomal band 19q13 in benign follicular thyroid tumors, we have used two cell lines derived from benign thyroid tumors showing translocations with 19q13 breakpoints for fluorescence in situ hybridization mapping studies with cosmid and PAC clones located in a 400-kbp region. The breakpoints of the chromosome 19 abnormalities mapped within a 140-kb segment covered by a single PAC clone. Sequencing of part of this PAC clone allowed us to establish the cDNA sequence and the genomic structure of a candidate gene located in close vicinity to the breakpoints. The gene that we tentatively refer to as RITA (rearranged in thyroid adenomas) belongs to the KRAB zinc finger protein coding genes. From our results we have concluded that in the two cell lines investigated the breaks have occurred either within the 5′ untranslated region of RITA or in its close 5′ vicinity. By Northern blot analyses two transcripts of about 4.7 kbp and 5 kbp were detected in normal thyroid tissue as well as in other normal tissues tested. An additional 2.1-kbp transcript was found only in testicular tissue. In contrast to all normal tissues, both cell lines with 19q aberrations expressed larger transcripts of approximately 5.5 kbp and 6.2 kbp. From the close vicinity to the breakpoint region, the expression patterns of the gene, and its type, we consider RITA a strong candidate target gene of the specific 19q aberrations in benign thyroid tumors. Genes Chromosomes Cancer 26:229–236, 1999. © 1999 Wiley-Liss, Inc. REFERENCES Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. 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