Spiral phase contrast imaging in microscopy
2005; Optica Publishing Group; Volume: 13; Issue: 3 Linguagem: Inglês
10.1364/opex.13.000689
ISSN1094-4087
AutoresSeverin Fürhapter, Alexander Jesacher, Stefan Bernet, Monika Ritsch‐Marte,
Tópico(s)Near-Field Optical Microscopy
ResumoWe demonstrate an optical method for edge contrast enhancement in light microscopy. The method is based on holographic Fourier plane filtering of the microscopic image with a spiral phase element (also called vortex phase or helical phase filter) displayed as an off-axis hologram at a computer controlled high resolution spatial light modulator (SLM) in the optical imaging pathway. The phase hologram imprints a helical phase term of the form exp(i phi) on the diffracted light field in its Fourier plane. In the image plane, this results in a strong and isotropic edge contrast enhancement for both amplitude and phase objects.
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