Direct Visualization of the Movement of a Single T7 RNA Polymerase and Transcription on a DNA Nanostructure
2012; Wiley; Volume: 124; Issue: 35 Linguagem: Inglês
10.1002/ange.201201890
ISSN1521-3757
AutoresMasayuki Endo, Koichi Tatsumi, Kosuke Terushima, Yousuke Katsuda, Kumi Hidaka, Yoshie Harada, Hiroshi Sugiyama,
Tópico(s)DNA and Nucleic Acid Chemistry
ResumoAngewandte ChemieVolume 124, Issue 35 p. 8908-8912 Zuschrift Direct Visualization of the Movement of a Single T7 RNA Polymerase and Transcription on a DNA Nanostructure† Dr. Masayuki Endo, Corresponding Author Dr. Masayuki Endo endo@kuchem.kyoto-u.ac.jp Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan) CREST, Japan Science and Technology Corporation (JST), Sanbancho, Chiyoda-ku, Tokyo 102-0075 (Japan)Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan)Search for more papers by this authorKoichi Tatsumi, Koichi Tatsumi Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan)Search for more papers by this authorKosuke Terushima, Kosuke Terushima Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan)Search for more papers by this authorYousuke Katsuda, Yousuke Katsuda Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan)Search for more papers by this authorKumi Hidaka, Kumi Hidaka Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan)Search for more papers by this authorProf. Yoshie Harada, Prof. Yoshie Harada Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan)Search for more papers by this authorProf. Hiroshi Sugiyama, Corresponding Author Prof. Hiroshi Sugiyama hs@kuchem.kyoto-u.ac.jp Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan) Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan) CREST, Japan Science and Technology Corporation (JST), Sanbancho, Chiyoda-ku, Tokyo 102-0075 (Japan)Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan)Search for more papers by this author Dr. Masayuki Endo, Corresponding Author Dr. Masayuki Endo endo@kuchem.kyoto-u.ac.jp Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan) CREST, Japan Science and Technology Corporation (JST), Sanbancho, Chiyoda-ku, Tokyo 102-0075 (Japan)Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan)Search for more papers by this authorKoichi Tatsumi, Koichi Tatsumi Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan)Search for more papers by this authorKosuke Terushima, Kosuke Terushima Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan)Search for more papers by this authorYousuke Katsuda, Yousuke Katsuda Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan)Search for more papers by this authorKumi Hidaka, Kumi Hidaka Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan)Search for more papers by this authorProf. Yoshie Harada, Prof. Yoshie Harada Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan)Search for more papers by this authorProf. Hiroshi Sugiyama, Corresponding Author Prof. Hiroshi Sugiyama hs@kuchem.kyoto-u.ac.jp Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan) Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan) CREST, Japan Science and Technology Corporation (JST), Sanbancho, Chiyoda-ku, Tokyo 102-0075 (Japan)Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan)Search for more papers by this author First published: 29 July 2012 https://doi.org/10.1002/ange.201201890Citations: 9 † This work was supported by Core Research for Evolutional Science and Technology (CREST) of JST and Grant-in-Aid for Scientific Research from MEXT (Japan). Financial support from Sumitomo foundation and Iketani Science and Technology Foundation to M.E. is also acknowledged. Read the full textAboutPDF ToolsRequest permissionAdd to favorites ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Graphical Abstract Schnappschüsse der Transkription: Die Bewegung eines T7-RNA-Polymerase(RNAP)-Moleküls entlang eines DNA-Doppelstrang(dsDNA)-Templats auf einer DNA-Origami-Plattform (grün, siehe Schema) wurde mit Hochgeschwindigkeits-AFM verfolgt. An einem dsDNA-Templat mit einer Länge von einer Kilobase, das den T7-Promotor enthielt, wurden AFM-Bilder von der Transkription durch die RNAP aufgenommen, und die produzierte biotinylierte RNA wurde durch Streptavidin-Markierung nachgewiesen. Citing Literature Supporting Information Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Filename Description ange_201201890_sm_miscellaneous_information.pdf1.8 MB miscellaneous_information ange_201201890_sm_movie_s1.wmv488 KB movie_s1 ange_201201890_sm_movie_s2.wmv396.2 KB movie_s2 ange_201201890_sm_movie_s3.wmv303.3 KB movie_s3 ange_201201890_sm_movie_s4.wmv528.1 KB movie_s4 Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume124, Issue35August 27, 2012Pages 8908-8912 This is the German version of Angewandte Chemie. Note for articles published since 1962: Do not cite this version alone. Take me to the International Edition version with citable page numbers, DOI, and citation export. We apologize for the inconvenience. RelatedInformation
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