Amperometric argentimetric and mercurimetric titration of sulfhydryl
1961; Elsevier BV; Volume: 24; Linguagem: Inglês
10.1016/0003-2670(61)80053-6
ISSN1873-4324
AutoresI. M. Kolthoff, J. Eisenstädter,
Tópico(s)Analytical chemistry methods development
ResumoThe ampero-mercurimetric titration of cysteine (RSH), glutathione (GSH), thioglycolic acid (TSH) and 2-mercaptoethanol (ESH) at the RMPE at pH 9 yields a sharply defined end-point corresponding to the composition (XS)2Hg. Under the same conditions with the KPtWK two lirciiks an; (ibst'rvc-d in the titration lines with RSH and GSH, corresponding to compositions (RS)2Hg and (RS)2Hg2 respectively. On the other hand, with TSHl and ESH only one clear break is found at the RPtWE corresponding to ('l'S)2Hg or (ES)2Hg Conditions liave been describcd for the accurate mercurimetric titration of the thiol compnunds at pH 2 in the pesence of 0.01 M sulfur dioxide at the RMPE, use of this can be made in the determination of disufides and in the determination of the equilibrium concentrations of the reaction betwcen a disulfide and a sulfite at varying pH. Les auteurs out efffectué une étude sur le titrage ampérométrique de la crystéine, de la glutathione, de P'acide thioglycolique et du mercapto-2-éthanol, par mercurimétrie. IIs ont (examiné également le comportement des disulfurcs et la détermination des concentrations d''equilibre, á divers pH, entre disulfure et sulfite. Beschreibung einer ampero-mercuirimetrischen Methode zur Bestimmung von Cystein, Glutathion, Thioglycolsäure und 2-Mercapto-ätlanol. Die Methode lässt sich verwenden zur Bestimmung von Disulfiden und Gleichgewichtskonzentrationen bei der Reaktion zwischen eineen Disulfid und Sulfit bei verschiedenem pH.
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