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Effects of leukapheresis protocol, cell processing and cryopreservation on the generation of monocyte-derived DC for immune therapy

2003; Elsevier BV; Volume: 5; Issue: 1 Linguagem: Inglês

10.1080/14653240310000056

1477-2566

Andrea Tazbirkova, Miki Okai, D. Horley, Tania Crough, Alyce Maksoud, Mie Nieda, Andrew J. Nicol,

RNA Interference and Gene Delivery

Background Many clinical trials of DC-based immunotherapy involve administration of monocyte-derived DCs (Mo-DC) on multiple occasions. We aimed to determine the optimal cell processing procedures and timing (leukapheresis, RBC depletion and cryopreservation) for generation of Mo-DC for clinical purposes. Methods Leukapheresis was undertaken using a COBE Spectra. Two instrument settings were compared - the standard semi-automated software (Version 4.7) (n =10) and the fully automated software (Version 6.0) (n =40). Density gradient centrifugation using Ficoll, Percoli, a combination of these methods or neither for RBC depletion were compared. Outcomes (including cell yield and purity) were compared for cryopreserved unmanipulated monocytes and cryopreserved Mo-DC. Results Software Version 6.0 provided significantly better enrichment for monocytes (P <0.05) but 25% fewer total monocytes. Final Mo-DC purity was not influenced by leultapheresis or RBC depletion method, but was critically dependent on monocyte adherence. Version 6.0 produced significantly lower RBC and platelet contamination (P <0.0005) but in vitro RBC depletion could not T'OIItineiy be omitted. Only 5–6% of monocytes harvested resulted in Mo-DC (95% lost in cell processing or foiling to differentiate). Discussion Cell losses remained significant despite attempts to minimise processing steps during Mo-DC generation. Reduction in RBC and platekts achieved with software version 6.0 was insufficient to offset the disadvantage of the lower monocyte yield. Substantial savings in materials and other costs can be achieved if Mo-DC for multiple treatments are generated from cryopreserved monocytes rather than from fresh monocytes.