HUman MicroNucleus project: international database comparison for results with the cytokinesis‐block micronucleus assay in human lymphocytes: I. Effect of laboratory protocol, scoring criteria, and host factors on the frequency of micronuclei
2001; Wiley; Volume: 37; Issue: 1 Linguagem: Inglês
10.1002/1098-2280(2001)37
ISSN1098-2280
AutoresStefano Bonassi, Michael Fenech, Cecilia Lando, Yi-Ping Lin, Marcello Ceppi, Wushou P. Chang, Nina Holland, Micheline Kirsch‐Volders, Errol Zeiger, Sadayuki Ban, Roberto Barale, Maria Paola Bigatti, Claudia Bolognesi, Jia Cao, Marina Di Giorgio, Lynnette R. Ferguson, Aleksandra Fučić, Omar García, Patrizia Hrelia, A.P. Krishnaja, Tung‐Kwang Lee, Lucia Migliore, Ludmilla Mikhalevich, E. Mirkova, Pasquale Mosesso, W Müller, Youichi Odagiri, Maria Rosaria Scarffi, Elena Szabová, Irena Vorobtsova, Anne Vral, Andrea Zijno,
Tópico(s)Acute Lymphoblastic Leukemia research
ResumoEnvironmental and Molecular MutagenesisVolume 37, Issue 1 p. 31-45 Research Article HUman MicroNucleus project: international database comparison for results with the cytokinesis-block micronucleus assay in human lymphocytes: I. Effect of laboratory protocol, scoring criteria, and host factors on the frequency of micronuclei Stefano Bonassi, Corresponding Author Stefano Bonassi bonassi@hp380.ist.unige.it Department of Environmental Epidemiology, Istituto Nazionale per la Ricerca sul Cancro, Genoa, ItalyIstituto Nazionale per la Ricerca sul Cancro, Department of Environmental Epidemiology, 10 Largo R. Benzi, Genoa I-16132, ItalySearch for more papers by this authorMichael Fenech, Michael Fenech Commonwealth Scientific and Industrial Research Organization (CSIRO) Health Sciences and Nutrition, Adelaide, AustraliaSearch for more papers by this authorCecilia Lando, Cecilia Lando Department of Environmental Epidemiology, Istituto Nazionale per la Ricerca sul Cancro, Genoa, ItalySearch for more papers by this authorYi-ping Lin, Yi-ping Lin Institute of Environmental Health Sciences, National Yang Ming University Medical School, Taipei, Taiwan, Republic of ChinaSearch for more papers by this authorMarcello Ceppi, Marcello Ceppi Department of Environmental Epidemiology, Istituto Nazionale per la Ricerca sul Cancro, Genoa, ItalySearch for more papers by this authorWushou Peter Chang, Wushou Peter Chang Institute of Environmental Health Sciences, National Yang Ming University Medical School, Taipei, Taiwan, Republic of ChinaSearch for more papers by this authorNina Holland, Nina Holland School of Public Health, University of California, Berkeley, CaliforniaSearch for more papers by this authorMicheline Kirsch-Volders, Micheline Kirsch-Volders Laboratory for Cell Genetics, Vrije Universiteit Brussels, Brussels, BelgiumSearch for more papers by this authorErrol Zeiger, Errol Zeiger Environmental Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, North CarolinaSearch for more papers by this authorSadayuki Ban, Sadayuki Ban Department of Radiobiology, Radiation Effects Research Foundation, Hiroshima, JapanSearch for more papers by this authorRoberto Barale, Roberto Barale Dipartimento di Scienze dell'Uomo e dell'Ambiente, University of Pisa, Pisa, ItalySearch for more papers by this authorMaria Paola Bigatti, Maria Paola Bigatti Dipartimento di Biologia Animale, University of Turin, Turin, ItalySearch for more papers by this authorClaudia Bolognesi, Claudia Bolognesi Toxicological Evaluation Unit, Istituto Nazionale per la Ricerca sul Cancro, Genoa, ItalySearch for more papers by this authorCao Jia, Cao Jia Molecular Toxicology Laboratory, Third Military Medical University, Chong Qing, ChinaSearch for more papers by this authorMarina Di Giorgio, Marina Di Giorgio Laboratorio Dosimetria Biologica, Autoridad Regulatoria Nuclear, Buenos Aires, ArgentinaSearch for more papers by this authorLynnette R. Ferguson, Lynnette R. Ferguson Auckland Cancer Society Research Centre, Auckland, New ZealandSearch for more papers by this authorAleksandra Fucic, Aleksandra Fucic Institute for Medical Research end Occupational Health, Zagreb, CroatiaSearch for more papers by this authorOmar Garcia Lima, Omar Garcia Lima Centro de Proteccion e Higiene de las Radiaciones, Havana, CubaSearch for more papers by this authorPatrizia Hrelia, Patrizia Hrelia Department of Pharmacology, University of Bologna, Bologna, ItalySearch for more papers by this authorAyyathan P. Krishnaja, Ayyathan P. Krishnaja Cell Biology Division, Bhabha Atomic Research Center, Mumbai, IndiaSearch for more papers by this authorTung-Kwang Lee, Tung-Kwang Lee Department of Radiation Oncology, Leo Jenkins Cancer Center, East Carolina University School of Medicine, Greenville, North CarolinaSearch for more papers by this authorLucia Migliore, Lucia Migliore Dipartimento di Scienze dell'Uomo e dell'Ambiente, University of Pisa, Pisa, ItalySearch for more papers by this authorLudmilla Mikhalevich, Ludmilla Mikhalevich Institute of Genetics and Cytology, Belarus Academy of Sciences, Minsk, BelarusSearch for more papers by this authorEkaterina Mirkova, Ekaterina Mirkova Nacional Center of Hygiene, Medical Ecology and Nutrition, Sofia, BulgariaSearch for more papers by this authorPasquale Mosesso, Pasquale Mosesso Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Viterbo, ItalySearch for more papers by this authorWolfgang-Ulrich Müller, Wolfgang-Ulrich Müller Institute für Medizinische Strahlenbiologie, Universitatsklinikum Essen, Essen, GermanySearch for more papers by this authorYouichi Odagiri, Youichi Odagiri Division of Human and Health Sciences, Yamanashi Prefectural College of Nursing, Kofu, JapanSearch for more papers by this authorMaria Rosaria Scarffi, Maria Rosaria Scarffi Consiglio Nazionale delle Ricerche (CNR)–Istituto Di Ricerca Per l'Elettromagnetismo ei Componendi Elettronici (IRECE), Naples, ItalySearch for more papers by this authorElena Szabova, Elena Szabova Institute of Preventive and Clinical Medicine, Bratislava, Slovak RepublicSearch for more papers by this authorIrena Vorobtsova, Irena Vorobtsova Central Research Institute of Roentgenology and Radiology, St. Petersburg, RussiaSearch for more papers by this authorAnne Vral, Anne Vral Department of Anatomy, Embryology, and Histology, University Gent, Ghent, BelgiumSearch for more papers by this authorAndrea Zijno, Andrea Zijno Laboratory of Comparative Toxicology and Ecotoxicology, Istituto Superiore di Sanità, Rome, ItalySearch for more papers by this author Stefano Bonassi, Corresponding Author Stefano Bonassi bonassi@hp380.ist.unige.it Department of Environmental Epidemiology, Istituto Nazionale per la Ricerca sul Cancro, Genoa, ItalyIstituto Nazionale per la Ricerca sul Cancro, Department of Environmental Epidemiology, 10 Largo R. Benzi, Genoa I-16132, ItalySearch for more papers by this authorMichael Fenech, Michael Fenech Commonwealth Scientific and Industrial Research Organization (CSIRO) Health Sciences and Nutrition, Adelaide, AustraliaSearch for more papers by this authorCecilia Lando, Cecilia Lando Department of Environmental Epidemiology, Istituto Nazionale per la Ricerca sul Cancro, Genoa, ItalySearch for more papers by this authorYi-ping Lin, Yi-ping Lin Institute of Environmental Health Sciences, National Yang Ming University Medical School, Taipei, Taiwan, Republic of ChinaSearch for more papers by this authorMarcello Ceppi, Marcello Ceppi Department of Environmental Epidemiology, Istituto Nazionale per la Ricerca sul Cancro, Genoa, ItalySearch for more papers by this authorWushou Peter Chang, Wushou Peter Chang Institute of Environmental Health Sciences, National Yang Ming University Medical School, Taipei, Taiwan, Republic of ChinaSearch for more papers by this authorNina Holland, Nina Holland School of Public Health, University of California, Berkeley, CaliforniaSearch for more papers by this authorMicheline Kirsch-Volders, Micheline Kirsch-Volders Laboratory for Cell Genetics, Vrije Universiteit Brussels, Brussels, BelgiumSearch for more papers by this authorErrol Zeiger, Errol Zeiger Environmental Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, North CarolinaSearch for more papers by this authorSadayuki Ban, Sadayuki Ban Department of Radiobiology, Radiation Effects Research Foundation, Hiroshima, JapanSearch for more papers by this authorRoberto Barale, Roberto Barale Dipartimento di Scienze dell'Uomo e dell'Ambiente, University of Pisa, Pisa, ItalySearch for more papers by this authorMaria Paola Bigatti, Maria Paola Bigatti Dipartimento di Biologia Animale, University of Turin, Turin, ItalySearch for more papers by this authorClaudia Bolognesi, Claudia Bolognesi Toxicological Evaluation Unit, Istituto Nazionale per la Ricerca sul Cancro, Genoa, ItalySearch for more papers by this authorCao Jia, Cao Jia Molecular Toxicology Laboratory, Third Military Medical University, Chong Qing, ChinaSearch for more papers by this authorMarina Di Giorgio, Marina Di Giorgio Laboratorio Dosimetria Biologica, Autoridad Regulatoria Nuclear, Buenos Aires, ArgentinaSearch for more papers by this authorLynnette R. Ferguson, Lynnette R. Ferguson Auckland Cancer Society Research Centre, Auckland, New ZealandSearch for more papers by this authorAleksandra Fucic, Aleksandra Fucic Institute for Medical Research end Occupational Health, Zagreb, CroatiaSearch for more papers by this authorOmar Garcia Lima, Omar Garcia Lima Centro de Proteccion e Higiene de las Radiaciones, Havana, CubaSearch for more papers by this authorPatrizia Hrelia, Patrizia Hrelia Department of Pharmacology, University of Bologna, Bologna, ItalySearch for more papers by this authorAyyathan P. Krishnaja, Ayyathan P. Krishnaja Cell Biology Division, Bhabha Atomic Research Center, Mumbai, IndiaSearch for more papers by this authorTung-Kwang Lee, Tung-Kwang Lee Department of Radiation Oncology, Leo Jenkins Cancer Center, East Carolina University School of Medicine, Greenville, North CarolinaSearch for more papers by this authorLucia Migliore, Lucia Migliore Dipartimento di Scienze dell'Uomo e dell'Ambiente, University of Pisa, Pisa, ItalySearch for more papers by this authorLudmilla Mikhalevich, Ludmilla Mikhalevich Institute of Genetics and Cytology, Belarus Academy of Sciences, Minsk, BelarusSearch for more papers by this authorEkaterina Mirkova, Ekaterina Mirkova Nacional Center of Hygiene, Medical Ecology and Nutrition, Sofia, BulgariaSearch for more papers by this authorPasquale Mosesso, Pasquale Mosesso Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Viterbo, ItalySearch for more papers by this authorWolfgang-Ulrich Müller, Wolfgang-Ulrich Müller Institute für Medizinische Strahlenbiologie, Universitatsklinikum Essen, Essen, GermanySearch for more papers by this authorYouichi Odagiri, Youichi Odagiri Division of Human and Health Sciences, Yamanashi Prefectural College of Nursing, Kofu, JapanSearch for more papers by this authorMaria Rosaria Scarffi, Maria Rosaria Scarffi Consiglio Nazionale delle Ricerche (CNR)–Istituto Di Ricerca Per l'Elettromagnetismo ei Componendi Elettronici (IRECE), Naples, ItalySearch for more papers by this authorElena Szabova, Elena Szabova Institute of Preventive and Clinical Medicine, Bratislava, Slovak RepublicSearch for more papers by this authorIrena Vorobtsova, Irena Vorobtsova Central Research Institute of Roentgenology and Radiology, St. Petersburg, RussiaSearch for more papers by this authorAnne Vral, Anne Vral Department of Anatomy, Embryology, and Histology, University Gent, Ghent, BelgiumSearch for more papers by this authorAndrea Zijno, Andrea Zijno Laboratory of Comparative Toxicology and Ecotoxicology, Istituto Superiore di Sanità, Rome, ItalySearch for more papers by this author First published: 31 January 2001 https://doi.org/10.1002/1098-2280(2001)37:1 3.0.CO;2-PCitations: 299AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Micronucleus (MN) expression in peripheral blood lymphocytes is well established as a standard method for monitoring chromosome damage in human populations. The first results of an analysis of pooled data from laboratories using the cytokinesis-block micronucleus (CBMN) assay and participating in the HUMN (HUman MicroNucleus project) international collaborative study are presented. The effects of laboratory protocol, scoring criteria, and host factors on baseline micronucleated binucleate cell (MNC) frequency are evaluated, and a reference range of “normal” values against which future studies may be compared is provided. Primary data from historical records were submitted by 25 laboratories distributed in 16 countries. This resulted in a database of nearly 7000 subjects. Potentially significant differences were present in the methods used by participating laboratories, such as in the type of culture medium, the concentration of cytochalasin-B, the percentage of fetal calf serum, and in the culture method. Differences in criteria for scoring micronuclei were also evident. The overall median MNC frequency in nonexposed (i.e., normal) subjects was 6.5‰ and the interquartile range was between 3 and 12‰. An increase in MNC frequency with age was evident in all but two laboratories. The effect of gender, although not so evident in all databases, was also present, with females having a 19% higher level of MNC frequency (95% confidence interval: 14–24%). Statistical analyses were performed using random-effects models for correlated data. Our best model, which included exposure to genotoxic factors, host factors, methods, and scoring criteria, explained 75% of the total variance, with the largest contribution attributable to laboratory methods. Environ. Mol. Mutagen. 37:31–45, 2001 © 2001 Wiley-Liss, Inc. Citing Literature Volume37, Issue12001Pages 31-45 RelatedInformation
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