A novel ubiquitin-specific protease is dynamically associated with the PML nuclear domain and binds to a herpesvirus regulatory protein
1997; Springer Nature; Volume: 16; Issue: 3 Linguagem: Inglês
10.1093/emboj/16.3.566
ISSN1460-2075
Autores Tópico(s)Cutaneous lymphoproliferative disorders research
ResumoArticle1 February 1997free access A novel ubiquitin-specific protease is dynamically associated with the PML nuclear domain and binds to a herpesvirus regulatory protein Roger D. Everett Roger D. Everett Medical Research Council Virology Unit, Church Street, Glasgow, G11 5JR UK Search for more papers by this author Michayla Meredith Michayla Meredith Medical Research Council Virology Unit, Church Street, Glasgow, G11 5JR UK Search for more papers by this author Anne Orr Anne Orr Medical Research Council Virology Unit, Church Street, Glasgow, G11 5JR UK Search for more papers by this author Anne Cross Anne Cross Medical Research Council Virology Unit, Church Street, Glasgow, G11 5JR UK Search for more papers by this author Meeta Kathoria Meeta Kathoria Medical Research Council Virology Unit, Church Street, Glasgow, G11 5JR UK Search for more papers by this author Jane Parkinson Jane Parkinson Medical Research Council Virology Unit, Church Street, Glasgow, G11 5JR UK Search for more papers by this author Roger D. Everett Roger D. Everett Medical Research Council Virology Unit, Church Street, Glasgow, G11 5JR UK Search for more papers by this author Michayla Meredith Michayla Meredith Medical Research Council Virology Unit, Church Street, Glasgow, G11 5JR UK Search for more papers by this author Anne Orr Anne Orr Medical Research Council Virology Unit, Church Street, Glasgow, G11 5JR UK Search for more papers by this author Anne Cross Anne Cross Medical Research Council Virology Unit, Church Street, Glasgow, G11 5JR UK Search for more papers by this author Meeta Kathoria Meeta Kathoria Medical Research Council Virology Unit, Church Street, Glasgow, G11 5JR UK Search for more papers by this author Jane Parkinson Jane Parkinson Medical Research Council Virology Unit, Church Street, Glasgow, G11 5JR UK Search for more papers by this author Author Information Roger D. Everett1, Michayla Meredith1, Anne Orr1, Anne Cross1, Meeta Kathoria1 and Jane Parkinson1 1Medical Research Council Virology Unit, Church Street, Glasgow, G11 5JR UK The EMBO Journal (1997)16:566-577https://doi.org/10.1093/emboj/16.3.566 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Herpes simplex virus type 1 immediate-early protein Vmw110 is a non-specific activator of gene expression and is required for efficient initiation of the viral lytic cycle. Since Vmw110-deficient viruses reactivate inefficiently in mouse latency models it has been suggested that Vmw110 plays a role in the balance between the latent and lytic states of the virus. The mechanisms by which Vmw110 achieves these functions are poorly understood. Vmw110 migrates to discrete nuclear structures (ND10) which contain the cellular PML protein, and in consequence PML and other constituent proteins are dispersed. In addition, Vmw110 binds to a cellular protein of ∼135 kDa, and its interactions with the 135 kDa protein and ND10 contribute to its ability to stimulate gene expression and viral lytic growth. In this report we identify the 135 kDa protein as a novel member of the ubiquitin-specific protease family. The protease is distributed in the nucleus in a micropunctate pattern with a limited number of larger discrete foci, some of which co-localize with PML in ND10. At early times of virus infection, the presence of Vmw110 increases the proportion of ND10 which contain the ubiquitin-specific protease. These results identify a novel, transitory component of ND10 and implicate a previously uncharacterized ubiquitin-dependent pathway in the control of viral gene expression. Introduction Herpes simplex virus type 1 (HSV-1) is a common human pathogen which attains a life-long latent state in sensory neurones after initial infection at the periphery. The establishment of latency and the subsequent episodes of reactivation are fundamental to the clinical importance of herpes simplex viruses and undoubtedly contribute substantially to their evolutionary success, as latency allows the virus to evade the immune system. The pattern of viral gene expression during lytic infection, when at least 76 genes are expressed from the 152 kb genome (McGeoch et al., 1993 and references therein), contrasts with that of latency when only one active viral transcription unit of unknown function has been detected (for a review see Fraser et al., 1992). HSV-1 genes can be divided into Immediate-Early (IE), Early and Late temporal classes depending on their time-course of synthesis and requirements for prior viral gene expression and DNA replication (reviewed by Roizman and Sears, 1990). Transcription of the IE genes is stimulated by the viral tegument protein VP16, which interacts with Oct-1 and other cellular proteins (Wilson et al., 1993 and references therein), and in turn four of the five IE gene products have roles in the normal expression of later classes of viral genes. The product of IE gene 3 is the major viral transcriptional transactivator Vmw175 (also known as ICP4), which forms a tripartite complex with TFIID and TFIIB to stimulate transcription from early and late promoters (Smith et al., 1993). IE gene 2 encodes Vmw63 (ICP27), which most likely acts at the post-transcriptional level to influence fully efficient expression of late genes (McCarthy et al., 1989; Sandri-Goldin and Mendoza, 1992; Phelan et al., 1993). Vmw68 (ICP22), the product of IE gene 4, is required for normal late gene expression in some cell lines and its presence results in the appearance of an underphosphorylated form of RNA polymerase II (Poffenberger et al., 1993; Rice et al., 1995). The product of IE gene 1 is the RING finger protein Vmw110 (ICP0), a strong and promiscuous activator of gene expression in transfection assays which can act synergistically with Vmw175 (reviewed by Everett et al., 1991). Several lines of evidence indicate that Vmw110 might have a specific role in influencing the latent–lytic switch. Viruses with lesions which inactivate Vmw110 can be propagated in culture, but they exhibit a marked multiplicity-dependence for the onset of lytic infection such that their probability of initiating a productive infection is much reduced (Stow and Stow, 1986; Sacks and Schaffer, 1987). The Vmw110-mutant virus particles which fail to begin replication enter a quiescent state from which they can be reactivated by superinfection (Stow and Stow, 1989). Tissue culture systems have been developed in which HSV-1 genomes can be established in a quiescent or latent state, and their reactivation can be induced by provision of exogenous Vmw110 (Harris et al., 1989; Zhu et al., 1990). Furthermore, Vmw110-deficient viruses reactivate inefficiently in mouse latency models (Clements and Stow, 1989; Lieb et al., 1989; Cai et al., 1993). Accordingly, it has been suggested that Vmw110 plays a role in the balance between the latent and lytic states; in its presence, the latter is favoured. The mechanisms by which Vmw110 achieves these functions are poorly understood. It is likely that cell factors also play major roles in controlling the replication status of the virus. For example, the multiplicity-dependent defect of Vmw110-deficient viruses can be modulated by both cell type and cell cycle status (Cai and Schaffer, 1991; Yao and Schaffer, 1995). Therefore, interactions between the virus and the cell, and in particular between Vmw110 and cellular proteins, are likely to be highly important for the biology of the virus. Two such interactions have been described; first, that between Vmw110 and the PML-containing nuclear bodies, and secondly, the direct binding of Vmw110 to a cellular protein of ∼135 kDa. Nuclear bodies (also known as PODs and referred to herein as ND10) are punctate structures associated with the nuclear matrix which have been shown to contain at least four different proteins (Ascoli and Maul, 1991; Stuurman et al., 1992; Dyck et al., 1994; Korioth et al., 1995). One of these proteins is PML, a RING finger protein which becomes fused to the retinoic acid receptor alpha (RARα) as a result of the t(15;17) chromosomal translocation in promyelocytic leukaemic blasts (de The et al., 1991; Goddard et al., 1991; Kakizuka et al., 1991; Kastner et al., 1992; Pandolfi et al., 1992). A consequence of this translocation is the disruption of ND10 from their normal appearance of an average of ∼10 punctate foci per nucleus, such that multiple small nuclear and cytoplasmic speckles are formed (Dyck et al., 1994; Koken et al., 1994; Weis et al., 1994). Since treatment with retinoic acid restores the normal ND10 staining pattern and results in differentiation of the blasts, ND10 have been suggested to play a role in the regulation of cell growth and proliferation. Interestingly, recent work has shown that ND10 are modified during infection by a number of DNA viruses (Maul et al., 1993; Kelly et al., 1995; Doucas et al., 1996). In the case of HSV-1, Vmw110 migrates to ND10 in the early stages of infection, and in consequence PML and other constituent proteins are dispersed (Everett and Maul, 1994; Maul and Everett, 1994). It is intriguing that mutations in Vmw110 that affect its roles in gene expression and the initiation of viral infection also alter the interaction of Vmw110 with ND10, thus implying that ND10 are involved in the onset of HSV-1 gene expression and infection. Indeed, it has been recently shown that ND10 are preferred locations within the nucleus where both HSV-1 and adenovirus DNA replication begin (Ishov and Maul, 1996; Maul et al., 1996). To investigate the molecular basis of the functions of Vmw110, we searched for cellular proteins to which it might bind and we found a strong and specific interaction between Vmw110 and a cellular protein of ∼135 kDa (Meredith et al., 1994, 1995). Deletion of the region of Vmw110 which binds to the 135 kDa protein reduces significantly its ability to stimulate gene expression and viral lytic growth (Everett, 1988; Meredith et al., 1995), and a deletion which overlaps this region eliminates the ability of Vmw110 to reactivate latent virus in an in vitro latency system (Zhu et al., 1990). In this report we identify the Vmw110-associated 135 kDa protein as a novel member of the ubiquitin-specific protease family which can be found associated with a subset of ND10. The expression of Vmw110 at early times of virus infection results in the presence of the protease in a higher proportion of ND10. These results define a novel transitory component of ND10 and implicate a previously uncharacterized ubiquitin-dependent pathway in both the function of ND10 and the control of viral gene expression. Results Cloning of a cDNA encoding a cellular Vmw110-associated protein We have shown that glutathione S-transferase (GST) fusion proteins containing the C-terminal 180 residues of Vmw110 bind to a cellular protein of ∼135 kDa in unfractionated cell extracts, and that a protein of identical gel mobility co-immunoprecipitates with Vmw110 from virus-infected cells (Meredith et al., 1994, 1995). In both assays, residues 594–633 of Vmw110 were found to be essential for this interaction, and because these residues also contribute to the functional and biological properties of Vmw110 (Everett, 1988; Meredith et al., 1995) it was considered of interest to identify the interacting cellular protein. The 135 kDa protein was purified by large-scale GST pull-down experiments using a GST fusion protein including residues 594–775 of Vmw110, and six internal peptide sequences were obtained (see Figure 1). An oligonucleotide based on peptide 45 was used to screen a HeLa cDNA library, and a family of overlapping cDNA clones was isolated. Sequence analysis revealed an open reading frame of 1102 codons, encoding a protein of predicted molecular weight 128 kDa, which included close or exact matches to all six of the original peptide sequences (Figure 1). Figure 1.Predicted amino acid sequence of HAUSP. The positions of the six peptide sequences derived from the HAUSP protein sample are marked; the discrepancies between the experimental peptide sequence data and the translated cDNA sequences are probably due to difficulties in sequencing the small amount of material that was available. The highly conserved residues within the cysteine and histidine motifs of the USP family of proteins are indicated, with O depicting conserved hydrophobic residues. Download figure Download PowerPoint A number of independent cDNA clones were obtained such that the majority of the predicted coding sequence was confirmed in at least two clones. The extreme 3′ end sequences were identified by a match of peptide 39 with an entry in the NCBI dbest database, and an RT–PCR clone was isolated which linked this sequence to the cloned cDNAs. The true 5′ end of the mRNA remains to be identified, but the presumed initiating methionine is likely to be correct as there are no further ATG triplets in over 200 bp of sequence further 5′ that is available. Additionally, this presumed 5′ untranslated leader region is exceptionally GC-rich (an average of 90%) which indicates that it is highly unlikely to be protein-coding. Three independent cDNA clones spanning at least part of this leader were isolated, which proves that these unusual sequences are not a cloning artefact. Analysis of the cDNA sequence Comparison of the protein sequence with the NCBI nr database showed that the Vmw110-associated protein contained excellent matches to the two conserved motifs of the ubiquitin-specific protease (USP) family (Baker et al., 1992; Papa and Hochstrasser, 1993; Figure 2) and accordingly we have renamed the protein HAUSP (herpesvirus-associated ubiquitin-specific protease). Sequences outside these motifs were not highly related to other USP enzymes of mammalian origin, but HAUSP has significant similarity throughout its sequence to uncharacterized proteins encoded by Saccharomyces cerevisiae (SC9952X6; 59% similarity, 34% identity) and Schizosaccharomyces pombe (Q09879; 57% similarity, 35% identity, where data are available) (Figure 2 and data not shown). This implies that HAUSP has homologues widely conserved through eukaryotic evolution. Figure 2.Compilation of the cysteine (A) and histidine (B) conserved domains of HAUSP and 20 members of the ubiquitin protease protein family. Proteins were detected by homology to HAUSP by the Blast program and the domain sequences were aligned using Pileup (GCG) before manual editing. The origins of the proteins are shown in (A), and the sequences are presented in the same order in (B). Further details of the selected proteins may be obtained from the quoted accession numbers. The consensus shows highly conserved residues that are present in HAUSP in bold; those that are not present in HAUSP are shown in lower case. Positions where hydrophobic residues are conserved are indicated by O. Note that there are groups of proteins which are more highly related to each other than to the family as a whole. Download figure Download PowerPoint Apart from the USP active site domains, database comparisons did not detect any other highly conserved features within the HAUSP sequence. However, of interest is a polyglutamine tract near the N-terminus, and several regions of high predicted α helix content, including the N-terminal 19 residues and segments between residues 515 and 572, 920 and 960, and 1008 and 1043. The first three of these regions display low-level similarity to the helical bundles of the involucrin family of proteins, which form an extended flexible rod and are thought to allow multiple intermolecular interactions (Yaffe et al., 1992). It is possible that the helical regions in HAUSP are involved in protein–protein binding events which influence the target specificity of the enzyme. Analysis of the HAUSP transcript To analyse the abundance of the HAUSP transcript, Northern blotting was performed using probes containing a 1.3 kb fragment at the 5′ end of the coding region and a 2 kb fragment further 3′. Both gave the same results, detecting two transcripts, one of ∼4.5 kb and a slightly larger (perhaps alternatively spliced) band of lesser abundance (Figure 3). These transcripts were only detectable in poly(A)+ RNA preparations and their low abundance (estimated by phosphorimager analysis of the blots as being at most 1% of that of γ-actin) emphasizes the specificity of the Vmw110–HAUSP interaction. Screening of the NCBI dbest database of expressed sequence tags revealed entries with precise matches in cDNA libraries derived from brain, liver, placenta, lung and melanocyte human cells. This suggests that HAUSP is expressed in a wide variety of cell types. Figure 3.Northern blot of the HAUSP transcript. Poly(A)+ selected and total cytoplasmic RNA samples were probed with RT–PCR clone MRMF15. The positions of the 28S and 18S ribosomal RNAs were determined in comparison with the stained gel and probing with a clone containing 28S rRNA sequences. Download figure Download PowerPoint HAUSP is enzymatically active on model substrates The ability of HAUSP to cleave model ubiquitin fusion protein substrates was investigated by co-expression in Escherichia coli. Complete coding region clones for HAUSP were constructed in T7-driven vectors in both pBR322 and pACYC184-based replicons (see Materials and methods), and these plasmids were introduced into bacteria harbouring model substrate expression cassettes in compatible replicons. These bacteria expressed a full-length protein of identical gel mobility to that of HeLa cell HAUSP both before induction and in increased amounts after induction of expression with IPTG (data not shown). As a control, plasmids expressing the yeast ubiquitin-specific protease UBP2 were analysed in parallel. A model substrate comprising the natural human Ub52 fusion protein precursor linked to GST, such that the ubiquitin sequences comprise the middle portion of the hybrid protein, was cleaved efficiently by UBP2 to yield a product of the expected size. Expression of HAUSP resulted in the same cleavage product, albeit at a reduced efficiency (Figure 4A). Similarly, both UBP2 and HAUSP cleaved the Ub-Met-β-galactosidase model substrate, although HAUSP was again less active (Figure 4B). It is not known whether the reduced activity of HAUSP in these assays is a consequence of relative expression levels, or whether it is a reflection of substrate specificity. Figure 4.Cleavage of model ubiquitin fusion protein substrates by HAUSP. (A) Bacteria harbouring plasmids expressing the indicated proteins (see Materials and methods) were grown up and expression of HAUSP or the positive control yeast UBP2 enzyme was either induced or not by the addition of IPTG (lanes marked + and − respectively). MW indicates molecular weight markers, the upper band (ovalbumin; 45 kDa) co-migrating with the predicted 42 kDa GST–Ub52 fusion protein, while the most prominent band in the MW lane is carbonic anhydrase, 29 kDa. The predicted size of the observed GST–Ub specific cleavage product is 36 kDa. (B) A Western blot of β-galactosidase proteins expressed by bacteria harbouring plasmids expressing the Ub–Met–β-gal model substrate and either HAUSP or UBP2, as marked. Expression of the USP enzymes in parallel cultures was either induced by the addition of IPTG or left uninduced (as indicated by + and −). The position of the uncleaved substrate is indicated by a dot in the second pair of tracks, while the correctly cleaved Met–β-gal product (as defined by the UBP2-positive control in the fourth pair of tracks) is indicated by a dot next to the third pair. Constitutive expression of UBP2 in this experiment is sufficient to cleave completely the low levels of substrate present. The more prominent and highly induced band of slightly higher gel mobility than the Met–β-gal product band is the truncated form of β-galactosidase expressed by the Novagen Blue (DE3) bacteria. Download figure Download PowerPoint Analysis of the Vmw110–HAUSP interaction Because the HAUSP cDNAs had been cloned on the basis of peptide sequences derived from a GST pull-down experiment using a segment of Vmw110, it was important to prove that the cloned cDNA encodes the protein which binds to Vmw110 in virus-infected cells. A portion of the HAUSP open reading frame was expressed in E.coli as a GST fusion protein and used to produce an anti-HAUSP rabbit antiserum (r29). This serum detected a band of the correct gel mobility in Western blots of HeLa cell extracts, and in addition two other bands which were also detected by the corresponding pre-immune serum (data not shown). Extracts of virus-infected cells were prepared and incubated with anti-Vmw110 Mab 11060 to immunoprecipitate Vmw110 and associated proteins. As previously reported, Vmw110 was immunoprecipitated with a cellular radiolabelled band of ∼135 kDa from wild-type virus-infected extracts, but the cellular protein was undetectable when mutant viruses D12 and E52X were used (Figure 5B). Viruses D12 and E52X have deletions which remove Vmw110 codons 594–633 and 594–775 respectively, thus deleting sequences that have been implicated in HAUSP binding (Meredith et al., 1994, 1995). Probing of this blot with r29 serum clearly indicated that the radiolabelled, co-immunoprecipitated band was indeed HAUSP (Figure 5A). A control probing the same blot with an anti-Vmw110 rabbit serum showed that Vmw110 expression and immunoprecipitation was equivalent in all three virus infections (Figure 5C). Figure 5.Vmw110 co-immunoprecipitates from virus-infected cells in complex with HAUSP. Immune precipitates using anti-Vmw110 Mab 11060 were prepared from mock-infected HeLa cells or cells infected with viruses 17+, D12 or E52X as indicated. The samples were Western blotted and probed with r29 serum (A). The blots were stripped and re-probed with anti-Vmw110 rabbit serum r95 to detect precipitated Vmw110 (C), then stripped again to detect labelled proteins by autoradiography (B). The HAUSP and Vmw110 proteins are arrowed. ex indicates tracks containing samples of the radiolabelled extracts used for the immunoprecipitations [which contain too little protein for HAUSP to be detected by r29 serum in (A)]; ip indicates tracks containing the immunoprecipitates. In a converse experiment, r29 serum was used to precipitate HAUSP from extracts of uninfected and infected cells prepared exactly as described above (lanes marked r29). The corresponding pre-immune serum was used as a control (lanes marked pi). Lanes marked ex contain labelled proteins from uninfected cells as a marker. After Western blotting, precipitated Vmw110 was detected with Mab 11060 (D); the blot was then stripped to detect radiolabelled proteins (E). The supernatants from the precipitations were analysed by Western blotting using Mab 11060 as a control for infection and expression of Vmw110 (F). Download figure Download PowerPoint In a converse experiment, r29 serum immunoprecipitated HAUSP from both uninfected and infected cells (Figure 5E). Probing the Western blot of these immunoprecipitation products with Mab 11060 showed that wild-type, but not mutant D12 or E52X Vmw110, was co-precipitated with HAUSP (Figure 5D). A control blot of the supernatants after the immunoprecipitations confirmed that infection and Vmw110 expression were equivalent in the three infected cell samples (Figure 5F). The amount of HAUSP precipitated from wild-type virus-infected cell extracts was reproducibly decreased compared with the other samples, indicating that the HAUSP epitopes recognized by r29 serum may be partially masked by the binding of Vmw110. These results confirm the specificity of r29 serum in immunoprecipitation reactions, and incontrovertibly establish that Vmw110 binds to the protein expressed by the cloned cDNA. It is worth noting that in some r29 immunoprecipitation experiments, trace amounts of mutant D12 Vmw110 were detected (data not shown, but it is extremely faintly visible in Figure 5D); perhaps the high affinity of Mab 11060 for Vmw110 gives a more sensitive assay, or the r29 antibodies stabilize a weak HAUSP–D12 interaction. This result suggests that the Vmw110 sequences which contact HAUSP have not been completely removed by the D12 deletion, which might explain why a virus with the D12 deletion grows more efficiently than a virus with the E52X deletion (Meredith et al., 1995). Further virus deletion mutants are currently under construction to investigate in more detail the precise Vmw110 sequence requirements for HAUSP binding. The r29 immunoprecipitation experiments reproducibly precipitated not only HAUSP but also a band of slightly higher molecular weight. It is possible that the larger band is an isoform of HAUSP, perhaps translated from the relatively minor higher molecular weight mRNA (Figure 3), or it could be a form with altered post-translational modifications. HAUSP is associated with a subset of ND10 Since Vmw110 binds to HAUSP and also localizes within the nucleus at the PML-containing ND10 structures, we investigated by immunofluorescence whether HAUSP was a normal component of ND10. Initial experiments used r29 serum, but consistent results of higher quality were obtained with r206 serum which was generated after immunization with a branched peptide containing HAUSP residues 1087–1102. Hep2 and human fetal lung (HFL) cells were co-stained with r206 and Mab 5E10 which detects PML (Stuurman et al., 1992; Dyck et al., 1994). The anti-HAUSP r206 serum gave a microspeckled nuclear staining pattern, excluding the nucleoli, with a small and variable number of brighter dots in some (but not all) cells, some of which co-localized with PML in ND10 (this was more easily seen in Hep2 cells) (Figure 6A–D). As a control, the corresponding pre-immune r206 serum gave very faint staining that was not concentrated in the nucleus (not shown), and which indicates that the staining pattern was specific for HAUSP and that channel overlap during fluorescence was undetectable. Additional confirmation of the specificity of r206 serum was obtained in combined immunoprecipitation and Western blotting experiments (analogous to Figure 5A–C) and by immunofluorescence of cells transfected with a HAUSP expression vector (data not shown). From these results we can conclude that HAUSP is a predominantly nuclear protein which is present in a minority of ND10. This suggests that there is a dynamic interaction between HAUSP and ND10 which may depend on some aspect of the status of the cell. Figure 6.HAUSP co-localizes with PML in a subset of ND10 in uninfected cells and more generally after infection. Uninfected Hep2 cells (A and B) and HFL cells (C and D) were co-stained with anti-PML Mab 5E10 (left-hand panels) and anti-HAUSP serum r206 (right-hand panels). In panels (E) to (L), HFL cells were infected with wild-type HSV-1 strain 17 (E–H) or Vmw110 RING finger mutant FXE (I–L), fixed 1 h after virus absorption and stained with anti-PML Mab 5E10 (E and I) or anti-Vmw110 Mab 11060 (G and K), simultaneously with anti-HAUSP serum r206 (right-hand panels). The left- and right-hand panels show the same fields of cells, which have been selected to illustrate the range of phenotypes observed after examination of several thousand cells. Although it was possible to find cells in the experiments illustrated in panels (E–H) which exhibited far more striking co-localization than the examples shown, these particular fields were selected as being a fair representation of the bulk of the population. Careful examination is required to confirm that most (but not all) the regions of greater anti-HAUSP staining co-localize with some (but not all) of the punctate anti-PML or anti-Vmw110 staining. The bar in panel (L) indicates 5 μm. Download figure Download PowerPoint There is precedence for a dynamic interaction of a protein with ND10 in that NDP52, while present in ND10 in some cell lines, only becomes associated with ND10 in HeLa and Cos cells after treatment with interferon (Korioth et al., 1995). It remains to be seen if the localization of HAUSP at ND10 can also be modulated by cytokines or other stimuli. Co-localization of HAUSP, Vmw110 and ND10 at early times of virus infection To investigate the effect of Vmw110 on the intranuclear distribution of HAUSP, HFL cells were infected with wild-type HSV-1 strain 17 and mutant derivative FXE. Virus FXE expresses a mutant form of Vmw110 lacking the RING finger domain (Everett, 1989), which eliminates its ability to disrupt ND10 but not its migration to ND10, thus resulting in a stable co-localization of Vmw110 and PML (Everett and Maul, 1994; Maul and Everett, 1994). In contrast, in a wild-type virus infection, Vmw110 transiently co-localizes with PML before the disruption of ND10 (Maul and Everett, 1994). Infected cells were co-stained with r206 serum to detect HAUSP and with either Mab 5E10 to detect PML or Mab 11060 to detect Vmw110. In a proportion of wild-type virus-infected cells, some r206 staining co-localized with PML (Figure 6E and F; most easily seen in the lower cell) and examination of large numbers of infected cells suggested that this partial co-localization was more extensive than that seen in uninfected cells (compare with Figure 6C and D). However, there was considerable variability from cell to cell, and the images have been selected to give a fair representation of this variability. When wild-type virus-infected cells were co-stained with r206 and Mab 11060, careful examination of the distribution of increased localized r206 staining in a proportio
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