Handling of Reactive Compounds for X-ray Structure Analysis
1987; American Chemical Society; Linguagem: Inglês
10.1021/bk-1987-0357.ch010
ISSN1947-5918
Autores Tópico(s)Enzyme Structure and Function
ResumoMethods have been developed that allow simple handling of highly reactive crystalline compounds in preparation for x-ray analysis. The essence of the method consists in protecting the crystals with a layer of hydrocarbon oil during manipulation. Crystals can be prepared by controlled atmosphere techniques, but thereafter all handling can be carried out in the open. Routine use of gas stream cooling (<140 K) during x-ray data collection has allowed long-term exposure with retention of crystal integrity, without the use of protective capillaries. Use of fast data acquisition methods allows determination of new structures in 24 hrs, or less, without use of other analytical techniques.
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