RAINBOW TROUT LIVER ACTIVATION SYSTEMS WITH THE AMES MUTAGENICITY TEST
1990; Wiley; Volume: 9; Issue: 9 Linguagem: Inglês
10.1897/1552-8618(1990)9[1183
ISSN1552-8618
Autores ResumoEnvironmental Toxicology and ChemistryVolume 9, Issue 9 p. 1183-1192 Article Rainbow trout liver activation systems with the ames mutagenicity test B. Thomas Johnson, B. Thomas Johnson National Fisheries Contaminant Research Center, U.S. Department of the Interior, Fish and Wildlife Service, Columbia, Missouri 65201Search for more papers by this author B. Thomas Johnson, B. Thomas Johnson National Fisheries Contaminant Research Center, U.S. Department of the Interior, Fish and Wildlife Service, Columbia, Missouri 65201Search for more papers by this author First published: September 1990 https://doi.org/10.1002/etc.5620090909Citations: 8AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract A poikilothermic metabolic activation system developed from liver homogenate of rainbow trout (Oncorhynchus mykiss, formerly Salmo gairdneri) was used in the Ames Salmonella/Mammalian Microsome Mutagenicity Test. Postmitochondrial fractions (S9) mediated four model promutagens – 2-aminoanthracene (2AA), 2-aminofluorene (2AF), benzo[a]pyrene (BaP) and 3-methylcholanthrene (3MC)–that require two different exogenous metabolic activation routes to form mutagens with Salmonella TA98 and TA100. The enzymatic activity of trout S9 was cytochrome P-450-like; it was heat labile and oxygen- and cofactor-dependent. Preincubation temperature significantly influenced the sensitivity of the fish-activated Ames test. Bacterial mutagenesis with trout activation significantly decreased as preincubation temperature increased; the optimum S9 activation temperature range for trout was 10 to 15°C compared with 37°C for the rat. The liquid-preincubation test was best adapted to the trout poikilothermic activation system; it was significantly more sensitive than the plate-incorporation test in detecting histidine revertants. The S9 activity of trout and rat was qualitatively similar in the Ames test: that is, both fractions metabolically activated 2AA, 2AF, BaP and 3MC to produce bacterial mutagenesis with Salmonella TA98 and TA100. The use of this ecologically relevant exogenous activation system in the short-term predictive genotoxicity testing of freshwater ecosystems is helpful in the assessment of potential hazards of chemical contaminants on fishery resources. References 1 Ames, B. N. 1979. Identifying environmental chemicals causing mutations and cancer. Science 204: 587–593. 2 Devoret, R. 1979. 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