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Movement-specific signaling is differentially distributed across motor cortex layer 5 projection neuron classes

2022; Cell Press; Volume: 39; Issue: 6 Linguagem: Inglês

10.1016/j.celrep.2022.110801

2639-1856

Stephen P. Currie, Julian J. Ammer, Brian Premchand, Joshua Dacre, Yufei Wu, Constantinos Eleftheriou, Matt Colligan, Thomas Clarke, Leah Mitchell, A. Aldo Faisal, Matthias H. Hennig, Ian Duguid,

Neurobiology and Insect Physiology Research

Motor cortex generates descending output necessary for executing a wide range of limb movements. Although movement-related activity has been described throughout motor cortex, the spatiotemporal organization of movement-specific signaling in deep layers remains largely unknown. Here we record layer 5B population dynamics in the caudal forelimb area of motor cortex while mice perform a forelimb push/pull task and find that most neurons show movement-invariant responses, with a minority displaying movement specificity. Using cell-type-specific imaging, we identify that invariant responses dominate pyramidal tract (PT) neuron activity, with a small subpopulation representing movement type, whereas a larger proportion of intratelencephalic (IT) neurons display movement-type-specific signaling. The proportion of IT neurons decoding movement-type peaks prior to movement initiation, whereas for PT neurons, this occurs during movement execution. Our data suggest that layer 5B population dynamics largely reflect movement-invariant signaling, with information related to movement-type being routed through relatively small, distributed subpopulations of projection neurons.