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Long-Read Metagenomics Improves the Recovery of Viral Diversity from Complex Natural Marine Samples

2022; American Society for Microbiology; Volume: 7; Issue: 3 Linguagem: Inglês

10.1128/msystems.00192-22

2379-5077

Asier Zaragoza-Solas, Jose M. Haro‐Moreno, Francísco Rodríguez‐Valera, Mario López‐Pérez,

Plant Virus Research Studies

We explored the vast genetic diversity of environmental viruses by using a combination of cellular metagenome (as opposed to virome) sequencing using high-fidelity long-read sequences (in this case, PacBio CCS). This approach resulted in the recovery of a representative sample of the viral population, and it performed better (more phage contigs, larger average contig size) than Illumina sequencing applied to the same sample.