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Intranasal vaccination of hamsters with a Newcastle disease virus vector expressing the S1 subunit protects animals against SARS-CoV-2 disease

2022; Nature Portfolio; Volume: 12; Issue: 1 Linguagem: Inglês

10.1038/s41598-022-13560-z

2045-2322

Manolo Fernández‐Díaz, Katherine Calderón, Aldo Rojas-Neyra, Vikram N. Vakharia, Ricardo Choque-Guevara, Ángela Montalván, Astrid Poma-Acevedo, Dora Rios-Matos, Andrés Agurto-Arteaga, María de Grecia Cauti-Mendoza, Norma Pérez-Martínez, Gisela Isasi-Rivas, Luis Tataje‐Lavanda, Yacory Sernaque-Aguilar, Freddy Ygnacio, Manuel Criollo-Orozco, Edison Huaccachi-Gonzalez, Elmer Delgado-Ccancce, Doris Villanueva-Pérez, Ricardo Montesinos, Kristel Gutiérrez-Manchay, Katherinne Pauyac-Antezana, Ingrid Ramirez-Ortiz, Stefany Quiñones-García, Yudith Cauna-Orocollo, Katherine Vallejos-Sánchez, Angela Rios-Angulo, Dennis Fernandez, Mario I. Salguedo-Bohorquez, Julio Ticona, Manolo Fernández-Sanchez, Eliana Icochea D., Luis A. Guevara-Sarmiento, Mirko Zimic, Andrés Agurto-Arteaga, Ricardo Antiparra, Manuel Ardiles-Reyes, Katherine Calderón, Yudith Cauna-Orocollo, María de Grecia Cauti-Mendoza, Naer Chipana-Flores, Ricardo Choque-Guevara, Xiomara Chunga-Girón, Manuel Criollo-Orozco, Lewis De La Cruz, Elmer Delgado-Ccancce, Nicolás E. Delgado-Pease, Christian Elugo-Guevara, Manolo Fernández‐Díaz, Manolo Fernández- Sánchez, Luis A. Guevara-Sarmiento, Kristel Gutiérrez-Manchay, Oscar Heredia-Almeyda, Edison Huaccachi Gonzalez, Pedro Huerta-Roque, Eliana Icochea D., Gisela Isasi-Rivas, Gabriel Jiménez-Avalos, Romina A. Juscamaita-Bartra, Abraham Licla-Inca, Ángela Montalván, Ricardo Montesinos, Dennis Fernandez, Adiana Ochoa-Ortiz, Gustavo E. Olivos-Ramírez, Erika Páucar-Montoro, Katherinne Pauyac-Antezana, Jose L. Perez-Martinez, Norma Pérez-Martínez, Astrid Poma-Acevedo, Stefany Quiñones-García, Ingrid Ramirez-Ortiz, Daniel Ramos‐Sono, Angela Rios-Angulo, Dora Rios-Matos, Aldo Rojas-Neyra, Yomara K. Romero, Mario I. Salguedo-Bohorquez, Yacory Sernaque-Aguilar, Patricia Sheen, Luis F. Soto, Luis Tataje‐Lavanda, Julio Ticona, Vikram N. Vakharia, Katherine Vallejos-Sánchez, A. Paula Vargas-Ruiz, Doris Villanueva-Pérez, Renzo G. Villena, Freddy Ygnacio, Mirko Zimic,

COVID-19 Clinical Research Studies

Abstract The coronavirus disease-19 (COVID-19) pandemic has already claimed millions of lives and remains one of the major catastrophes in the recorded history. While mitigation and control strategies provide short term solutions, vaccines play critical roles in long term control of the disease. Recent emergence of potentially vaccine-resistant and novel variants necessitated testing and deployment of novel technologies that are safe, effective, stable, easy to administer, and inexpensive to produce. Here we developed three recombinant Newcastle disease virus (rNDV) vectored vaccines and assessed their immunogenicity, safety, and protective efficacy against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in mice and hamsters. Intranasal administration of rNDV-based vaccine candidates elicited high levels of neutralizing antibodies. Importantly, the nasally administrated vaccine prevented lung damage, and significantly reduced viral load in the respiratory tract of vaccinated animal which was compounded by profound humoral immune responses. Taken together, the presented NDV-based vaccine candidates fully protected animals against SARS-CoV-2 challenge and warrants evaluation in a Phase I human clinical trial as a promising tool in the fight against COVID-19.