Uricase based amperometric biosensor improved by AuNPs-TiS2 nanocomposites for uric acid determination
2022; Elsevier BV; Volume: 181; Linguagem: Inglês
10.1016/j.microc.2022.107725
ISSN1095-9149
AutoresBaha Öndeş, Sinem Evli, Yüksel Şahin, Murat Uygun, Deniz Aktaş Uygun,
Tópico(s)Advanced biosensing and bioanalysis techniques
Resumo• AuNPs-TiS 2 nanocomposite modified SPE was prepared. • This prepared biosensor was used for the determination of uric acid. • This enzyme biosensor offered a wide measurement range and low LOD. • High selectivity for uric acid noticed in the presence of interfering agents. • Uric acid determination in commercial human serum was demonstrated. In this study, gold nanoparticles (AuNPs) as a zero-dimensional nanomaterial and TiS 2 nanosheets as a two-dimensional material and their composite were prepared and characterized. These AuNPs-TiS 2 nanocomposites interacted with uricase enzyme and Nafion™ and used to modify the working electrode of the Screen-Printed Electrode (SPE). This prepared nanocomposite modified biosensor was used for the determination of uric acid. Transmission electron microscopy (TEM) photos of the resulting nanocomposite showed that AuNPs were dispersed into TiS 2 nanosheets without aggregation. It was observed that the enzyme biosensor with the composite modification offered a wide measurement range (5–2000 µM) and exhibited low LOD (0.18 µM). The prepared uric acid biosensor has high sensitivity, low K m value, and fascinating storage stability. This uric acid biosensor showed high selectivity towards uric acid in the presence of urea, L-cysteine, glucose, and ascorbic acid. Additionally, this biosensor was successfully used for the determination of uric acid in commercial human serum with high recovery. This study revealed that the use of nanocomposites in biosensor design will yield impressive results.
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