Produção Nacional
Revisado por pares
Magnetite-levan nanoparticles for lectin purification: A single-step strategy for protein isolation from the seeds extract of the plant Cratylia mollis
2022; Elsevier BV; Volume: 1677; Linguagem: Inglês
10.1016/j.chroma.2022.463292
ISSN1873-3778
AutoresDaiane Laise da Silva, Mariana Paola Cabrera, Igor Teixeira Cavalcanti, Guilherme Rabelo Coelho, Emídio Beraldo-Neto, Rafael José Ribeiro Padilha, Carlos Eduardo Sales da Silva, Maria Tereza dos Santos Correia, Daniel C. Pimenta, Luiz Bezerra de Carvalho,
Tópico(s)Plant nutrient uptake and metabolism
ResumoThe ability to reversibly bind carbohydrates is an incredible property from lectins. Such characteristic has led these molecules to be employed in several applications involving medical research and biotechnology. Generally, these proteins follow several steps towards purification. Here, the synthesis, physical characterization, and use of levan-coated magnetite nanoparticles (MNPs-levan) for lectin isolation is described. Canavalia ensiformis and Cratylia mollis were used as sources of Concanavalin A and Cramoll, respectively, that were purified by using MNPs-levan. Mass spectrometry, SDS-PAGE, and hemagglutinating activity were employed to assess the efficiency of the process. Moreover, by using mass spectrometry approaches, a novel lectin, similar to Canavalin, was also identified for C. mollis, corroborating the advantages of using nanoparticles over microparticles. MNPs-levan could also be recycled, making this a low-cost, scalable process that can be efficiently employed over crude samples.
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