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Sensitive Detection of Heart-Failure Biomarkers Natriuretic Peptides Using Multi-Photon Laser Wave-Mixing Spectroscopy

2022; RELX Group (Netherlands); Linguagem: Inglês

10.2139/ssrn.4149581

1556-5068

James Suprapto, William G. Tong,

Spectroscopy Techniques in Biomedical and Chemical Research

Nonlinear laser wave-mixing spectroscopy is demonstrated as a fast and sensitive detection method for heart-failure biomarkers, pro-atrial natriuretic peptide (proANP) and brain natriuretic peptide (BNP). Wave mixing is an ultrasensitive optical absorption-based method and analytes can be detected in their native form or labeled with fluorophore and chromophore labels. We utilized Chromeo P540 dye to label the peptides for wave-mixing detection. In the presence of an absorbing sample, the wave-mixing signal is created from the diffraction of incoming photons by the thermal grating at the capillary analyte cell. The signal beam is strong, collimated, and coherent (laser-like) and it can be collected by a simple photodetector with an excellent signal-to-noise ratio. Advantages of this technique over conventional assays include shorter analysis times and smaller sample requirements, in addition to higher throughput analyses. To enhance detection selectivity and sensitivity levels, wave mixing can be effectively coupled with capillary zone electrophoresis (CZE) and field-amplified sample stacking (FASS) methods. Detection limits of 7.4 × 10-10 M or 55 zeptomole and 6.8 × 10-10 M or 51 zeptomole are determined for proANP and BNP, respectively. Due to the challenges in the confirmatory diagnoses of heart failure, wave-mixing serves as a potentially beneficial screening tool in addition to the commonly used echocardiographic tests.