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iChip-Inspired Isolation, Bioactivities and Dereplication of Actinomycetota from Portuguese Beach Sediments

2022; Multidisciplinary Digital Publishing Institute; Volume: 10; Issue: 7 Linguagem: Inglês

10.3390/microorganisms10071471

2076-2607

José Diogo Neves dos Santos, Susana Afonso João, Jesús Martı́n, Francisca Vicente, Fernando Reyes, Olga Maria Lage,

Marine Sponges and Natural Products

Oceans hold a stunning number of unique microorganisms, which remain unstudied by culture-dependent methods due to failures in establishing the right conditions for these organisms to grow. In this work, an isolation effort inspired by the iChip was performed using marine sediments from Memoria beach, Portugal. The isolates obtained were identified by 16S rRNA gene analysis, fingerprinted using BOX-PCR and ERIC-PCR, searched for the putative presence of secondary metabolism genes associated with polyketide synthase I (PKS-I) and non-ribosomal peptide synthetases (NRPS), screened for antimicrobial activity against