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Poly(lipoic acid)-based nanoparticles as a new therapeutic tool for delivering active molecules

2022; Elsevier BV; Volume: 45; Linguagem: Inglês

10.1016/j.nano.2022.102593

1549-9642

Chiara Castellani, Claudia Maria Radu, Lucía Morillas-Becerril, Ilaria Barison, Federica Menato, Tomaz Michele Do Nascimento, Marny Fedrigo, Alessia Giarraputo, Grazia Maria Virzì, Paolo Simioni, Cristina Basso, Emanuele Papini, Regina Tavano, Fabrizio Mancin, Giorgio Vescovo, Annalisa Angelini,

Macrophage Migration Inhibitory Factor

Pluronic-coated polylipoic acid-based nanoparticles (F127@PLA-NPs) have great potential as biodegradable nanovectors for delivering active molecules to different organs in complex diseases. In this study we describe the in vivo biodistribution, safety and ability to deliver molecules of F127@PLA-NPs in healthy rats following intravenous administration. Adult rats were injected with 10 mg/kg of rhodamine B-labeled F127@PLA-NPs, and NPs fluorescence and MFI rate were measured by confocal microscopy in whole collected organs. The NPs accumulation rate was maximal in the heart, compared to the other organs. At the cellular level, myocytes and kidney tubular cells showed the highest NPs uptake. Neither histopathological lesion nor thrombogenicity were observed after NPs injection. Finally, F127@PLA-NPs were tested in vitro as miRNAs delivery nanosystem, and they showed good ability in targeting cardiomyocytes. These results demonstrated that our F127@PLA-NPs constitute a biological, minimally invasive and safe delivery tool targeting organs and cells, such as heart and kidney.