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Functional Effects of Cardiomyocyte Injury in COVID-19

2021; American Society for Microbiology; Volume: 96; Issue: 2 Linguagem: Inglês

10.1128/jvi.01063-21

1098-5514

Mustafa M. Siddiq, Angel Chan, Lisa Miorin, Arjun Singh Yadaw, Kristin G. Beaumont, Thomas Kehrer, Anastasija Čupić, Kris M. White, Rosa E. Tolentino, Bin Hu, Alan D. Stern, Iman Tavassoly, Jens Hansen, Robert Sebra, Pedro Luis Véliz Martínez, S. Prabha, Nicole Dubois, Christoph Schaniel, Rupa Iyengar-Kapuganti, Nina Kukar, Gennaro Giustino, Karan Sud, Sharon Nirenberg, Patricia Kovatch, Randy A. Albrecht, Joseph Goldfarb, Lori B. Croft, Maryann McLaughlin, Edgar Argulian, Stamatios Lerakis, Jagat Narula, Adolfo García‐Sastre, Ravi Iyengar,

Long-Term Effects of COVID-19

COVID-19 affects multiple organs. Clinical data from the Mount Sinai Health System show that substantial numbers of COVID-19 patients without prior heart disease develop cardiac dysfunction. How COVID-19 patients develop cardiac disease is not known. We integrated cell biological and physiological analyses of human cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs) infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the presence of interleukins (ILs) with clinical findings related to laboratory values in COVID-19 patients to identify plausible mechanisms of cardiac disease in COVID-19 patients. We infected hiPSC-derived cardiomyocytes from healthy human subjects with SARS-CoV-2 in the absence and presence of IL-6 and IL-1β. Infection resulted in increased numbers of multinucleated cells. Interleukin treatment and infection resulted in disorganization of myofibrils, extracellular release of troponin I, and reduced and erratic beating. Infection resulted in decreased expression of mRNA encoding key proteins of the cardiomyocyte contractile apparatus. Although interleukins did not increase the extent of infection, they increased the contractile dysfunction associated with viral infection of cardiomyocytes, resulting in cessation of beating. Clinical data from hospitalized patients from the Mount Sinai Health System show that a significant portion of COVID-19 patients without history of heart disease have elevated troponin and interleukin levels. A substantial subset of these patients showed reduced left ventricular function by echocardiography. Our laboratory observations, combined with the clinical data, indicate that direct effects on cardiomyocytes by interleukins and SARS-CoV-2 infection might underlie heart disease in COVID-19 patients.