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De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

2022; Nature Portfolio; Volume: 13; Issue: 1 Linguagem: Inglês

10.1038/s41467-022-31746-x

2041-1723

Guillaume Tetreau, M.R. Sawaya, Elke De Zitter, Elena A. Andreeva, Anne‐Sophie Banneville, Natalie A. Schibrowsky, Nicolas Coquelle, Aaron S. Brewster, Marie Luise Grünbein, Gabriela Nass Kovács, Mark S. Hunter, Marco Kloos, Raymond G. Sierra, Giorgio Schirò, Pei Qiao, Myriam Stricker, Dennis K. Bideshi, I.D. Young, Ninon Zala, Sylvain Engilberge, Alexander Gorel, Luca Signor, Jean‐Marie Teulon, M. Hilpert, L. Foucar, Johan Bielecki, Richard Bean, Raphaël de Wijn, Tokushi Sato, Henry Kirkwood, Romain Letrun, A. Batyuk, I. Snigireva, Daphna Fenel, Robin Schubert, Ethan Canfield, Mario Alba, Fréderic Laporte, Laurence Després, Maria Bacia, Amandine Roux, Christian Chapelle, François Riobé, Olivier Maury, Wai Li Ling, Sébastien Boutet, Adrian P. Mancuso⋈, Irina Gutsche, Éric Girard, Thomas R. M. Barends, Jean‐Luc Pellequer, Hyunwoo Park, Arthur Laganowsky, José A. Rodríguez, Manfred Burghammer, Robert L. Shoeman, R. Bruce Doak, Martin H. Weik, Nicholas K. Sauter, Brian A. Federici, Duilio Cascio, Ilme Schlichting, Jacques‐Philippe Colletier,

Insect and Pesticide Research

Abstract Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan , respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable crystal packing motif is located within the protoxin rather than at the termini. The diversity of in vivo crystallization patterns suggests explanations for their varied levels of toxicity and rational approaches to improve these toxins for mosquito control.