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Biocatalytic routes to stereo-divergent iridoids

2022; Nature Portfolio; Volume: 13; Issue: 1 Linguagem: Inglês

10.1038/s41467-022-32414-w

2041-1723

Néstor J. Hernández Lozada, Benke Hong, Joshua C. Wood, Lorenzo Caputi, J. Basquin, Ling Chuang, Maritta Kunert, Carlos E. Rodríguez López, Chloe Langley, Dongyan Zhao, C. Robin Buell, Benjamin R. Lichman, Sarah E. O’Connor,

Photosynthetic Processes and Mechanisms

Abstract Thousands of natural products are derived from the fused cyclopentane-pyran molecular scaffold nepetalactol. These natural products are used in an enormous range of applications that span the agricultural and medical industries. For example, nepetalactone, the oxidized derivative of nepetalactol, is known for its cat attractant properties as well as potential as an insect repellent. Most of these naturally occurring nepetalactol-derived compounds arise from only two out of the eight possible stereoisomers, 7 S - cis - trans and 7 R - cis - cis nepetalactols. Here we use a combination of naturally occurring and engineered enzymes to produce seven of the eight possible nepetalactol or nepetalactone stereoisomers. These enzymes open the possibilities for biocatalytic production of a broader range of iridoids, providing a versatile system for the diversification of this important natural product scaffold.