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Antibacterial activity of Brazilian red propolis and in vitro evaluation of free radical production

2022; Elsevier BV; Volume: 143; Linguagem: Inglês

10.1016/j.archoralbio.2022.105520

1879-1506

Nilson Ferreira, Jéssica Fernanda Sena Bonvicini, Gabriela Leite de Souza, Mariana B. Santiago, Rodrigo Cássio Sola Veneziani, Sérgio Ricardo Ambrósio, Jairo Kenupp Bastos, Marcelo José Barbosa Silva, Carlos Henrique Gomes Martins, Camilla Christian Gomes Moura, Ana Paula Silveira Turrioni,

Endodontics and Root Canal Treatments

This study aimed to evaluate the antibacterial activity of crude Brazilian red propolis (BRP) extract against anaerobic bacteria involved in primary endodontic infection. Additionally, we evaluate the cell viability and free radical production of human dental pulp fibroblasts (HDPF) in direct contact with mineral trioxide aggregate (MTA) and BRP. The Minimum Inhibitory Concentration, Minimum Bactericidal Concentration (MIC, MBC) and Minimum Inhibitory Concentration of Biofilm (MICB50) of BRP against anaerobic endodontic pathogens were determined. HDPF were exposed to BRP10 (10 μg/mL), BRP50 (50 μg/mL), MTA extract (1:1, 1:2, 1:4 e 1:8), dimethyl sulfoxide 0.5% (DMSO), and cell culture medium (DMEM). The groups were tested for cell viability (MTT assay), and free radical production (reactive oxygen species - ROS, DCFH-DA probe and nitric oxide – NO, Griess reagent). The one-way ANOVA and Tukey's tests were employed at a significance level of 5%. MIC/MBC values of BRP performed antibacterial activity for Parvimonas micra (6.25/6.25 µg/mL), Fusobacterium nucleatum (25/25 µg/mL), Prevotella melaninogenica (50/100 µg/mL), Prevotella nigrescens (50/100 µg/mL), Prevotella intermedia (50/100 µg/mL), and Porphyromonas gingivalis (50/200 µg/mL). The MICB50 values ranged from 1.56 to 50 μg/mL. BRP and MTA stimulated cell viability, emphasizing BRP10 (p = 0.007). Furthermore, it was observed that MTA 1:1, MTA 1:2, and BRP50 slightly increased ROS (p < 0.001) and NO production (p = 0.008, p = 0.007, and p < 0.001 respectively) compared to DMEM group. BRP exhibits good antibacterial activity against endodontic pathogens, and both BRP and MTA promote the viability of HDPF without increasing NO and ROS production.