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First Report of Odontoglossum Ringspot Virus in Vanilla (Orchidaceae) in Madagascar

2022; American Phytopathological Society; Volume: 107; Issue: 4 Linguagem: Inglês

10.1094/pdis-06-22-1399-pdn

1943-7692

Mendrikaja Randriambololona, Tahinarizaka Jenny Rakotonirina, Adrien Rieux, Michel Grisoni,

Plant and animal studies

HomePlant DiseaseVol. 107, No. 4First Report of Odontoglossum Ringspot Virus in Vanilla (Orchidaceae) in Madagascar PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Odontoglossum Ringspot Virus in Vanilla (Orchidaceae) in MadagascarMendrikaja Randriambololona, Tahinarizaka Jenny Rakotonirina, Adrien Rieux, and Michel GrisoniMendrikaja RandriambololonaDepartment of Plant Biology and Ecology, University of Antananarivo, Antananarivo 101, MadagascarCentre Technique Horticole de Tamatave, Toamasina 501, MadagascarSearch for more papers by this author, Tahinarizaka Jenny RakotonirinaDepartment of Plant Biology and Ecology, University of Antananarivo, Antananarivo 101, MadagascarSearch for more papers by this author, Adrien RieuxCIRAD, UMR PVBMT, Saint Pierre 97410, La RéunionSearch for more papers by this author, and Michel Grisoni†Corresponding author: M. Grisoni; E-mail Address: [email protected]https://orcid.org/0000-0001-9867-9098CIRAD, UMR PVBMT, Toamasina 501, MadagascarSearch for more papers by this authorAffiliationsAuthors and Affiliations Mendrikaja Randriambololona1 2 Tahinarizaka Jenny Rakotonirina1 Adrien Rieux3 Michel Grisoni4 † 1Department of Plant Biology and Ecology, University of Antananarivo, Antananarivo 101, Madagascar 2Centre Technique Horticole de Tamatave, Toamasina 501, Madagascar 3CIRAD, UMR PVBMT, Saint Pierre 97410, La Réunion 4CIRAD, UMR PVBMT, Toamasina 501, Madagascar Published Online:7 Mar 2023https://doi.org/10.1094/PDIS-06-22-1399-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleVanilla (Vanilla planifolia, Orchidaceae) is Madagascar’s leading agricultural export resource, and the country provides 80% of the world’s consumption. During a phytosanitary survey from November 2019 to March 2021 in the main vanilla production regions of Madagascar, 250 plots were indexed for Cymbidium mosaic virus (CymMV, Potexvirus genus) and Odontoglossum ringspot virus (ORSV, Tobamovirus genus), the two most prevalent viruses of cultivated orchids worldwide (Zettler et al. 1990). For each plot, bulk samples (10 leaves taken at random) were assayed using ImmunoStrips (AGDIA, ISK 13301). A quarter of the plots (63/250) tested positive for CymMV. The highest prevalence of CymMV was observed in the SAVA region (57 out of 153 plots = 37.2%) where the virus has been reported since 1997 (Grisoni et al. 2010). Six plots in the district of Mahanoro (Atsinanana) tested positive for ORSV. A few plants in these plots showed chlorotic often annular spots on their leaves. They were individually tested positive for ORSV, and negative for CymMV and potyviruses (ImmunoStrips AGDIA ISK 27200), the other two viruses reported so far in vanilla in Madagascar. To confirm the diagnosis of ORSV, leaf samples from five of the six infected plots were analyzed by Tube Capture-RT-PCR (Grisoni et al. 2017) using two pairs of primers flanking the ORSV coat protein (CP) gene: OrCP1 (5′-GGTCGGTAATGGTGTTAG-3′)/OrCP2 (5′-TGCATTATCGTATGCTCC-3′), and CPOR-F (5′-ATGTCTTACACTATTACAGACC-3′)/CPOR-R (5′-TTAGGAAGAGGTCCAAGTAAG-3′). The five samples gave amplicons of the expected size (820 and 476 nt, respectively) and were sequenced with Sanger technology (Macrogen, The Netherlands). The ORSV-CP sequences of the Mahanoro isolates showed very close similarity to 198 ORSV-CP sequences from GenBank (95.8 to 99.6% nucleotide and 94.5 to 100% amino-acid identities), and less than 75.4% nucleotide (80.1% amino-acid) identities with bell pepper mosaic virus (DQ355023), the tobamovirus closest to ORSV. The five ORSV-CP sequences from vanilla were deposited in GenBank under accessions numbers OM847399 to OM847403. These data confirmed that ORSV infects vanilla vines in Madagascar. To our knowledge, this is the first report of this virus in Madagascar and of its ability to symptomatically infect V. planifolia. The five ORSV isolates from vanilla had more than 98.7% nucleotide identities of the CP gene and clustered into a monophyletic group in a maximum likelihood phylogenetic tree, suggesting a single origin of these isolates. To further investigate the origin of ORSV in Madagascar, we made use of RNA sequences isolated at different points in time to infer the timing of evolutionary events (Rieux and Balloux 2016). We estimated the CP gene substitution rate at 4.8E−4 subst,/site/year [95% HPD 2.1E−4 to 8.7E−4] which is close to the estimate of He et al. (2019) based on a slightly different sequences set (1.25E−3 subst./site/year). We dated the initial contamination of vanilla plots by ORSV between 2004 and 2013. Both ORSV and CymMV have deleterious effects on many ornamental orchids, and the pathogenicity of CymMV is exacerbated when coinfecting with ORSV (Lee et al. 2021). Therefore, ORSV represents a new threat to the Malagasy vanilla crop, especially in regions where CymMV is already rife. Given the economic importance of vanilla cultivation in the country, the implementation of prophylactic measures aimed at preventing the spread of ORSV, in particular through the sanitary control of cuttings, should be a priority for the vanilla industry.The author(s) declare no conflict of interest.References:Grisoni, M., et al. 2010. Page 95 in: Vanilla, Vol. 47. CRC Press, Boca Raton, FL. Google ScholarGrisoni, M., et al. 2017. Arch. Virol. 162:3855. https://doi.org/10.1007/s00705-017-3540-9 Crossref, ISI, Google ScholarHe, Z., et al. 2019. Plant Pathol. J. 35:498. https://doi.org/10.5423/PPJ.OA.04.2019.0113 Crossref, ISI, Google ScholarLee, S.-C., et al. 2021. Viruses 13:1552. https://doi.org/10.3390/v13081552 Crossref, ISI, Google ScholarRieux, A., and Balloux, F. 2016. Mol. Ecol. 25:1911. https://doi.org/10.1111/mec.13586 Crossref, ISI, Google ScholarZettler, F. W., et al. 1990. Plant Dis. 74:621. Crossref, ISI, Google ScholarFunding: This work was supported by the European Regional Development Fund (INTERREG V program) and the Conseil Régional de la Réunion.The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 107, No. 4 April 2023SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 27 Apr 2023Published: 7 Mar 2023First Look: 26 Aug 2022Accepted: 22 Aug 2022 Page: 1250 Information© 2023 The American Phytopathological SocietyFundingEuropean Regional Development FundConseil Régional de la RéunionKeywordsCymbidium mosaic virusetiologynucleotide substitution rateorchid virustropical plantsVanilla planifoliaviruses and viroidsThe author(s) declare no conflict of interest.PDF download