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Enhanced imaging of endogenous metabolites by negative ammonia assisted DESI/PI mass spectrometry

2022; Elsevier BV; Volume: 252; Linguagem: Inglês

10.1016/j.talanta.2022.123864

1873-3573

Zhan Li, Chengyuan Liu, Keke Qi, Liutian Wu, Ying Xiong, Xuan Zhang, Jianye Zang, Yang Pan,

Analytical Chemistry and Chromatography

In this work, endogenous metabolites in mouse brain tissue were imaged by negative desorption electrospray ionization (DESI), ammonia assisted DESI (aa-DESI), DESI/post-photoionization (DESI/PI), and ammonia assisted DESI/PI (aa-DESI/PI) mass spectrometry imaging (MSI) strategies. The combined effect of ammonia additive and post-photoionization was found to play an important role in the enhancement of sensitivity and coverage for endogenous analytes under ambient conditions. Compared with DESI, aa-DESI/PI can provide increased signal intensities for metabolites up to 37.1-fold, as well as the imaging of nine more small metabolites (m/z < 350) (26 for aa-DESI/PI and 17 for DESI) in mouse brain tissue. The results of Pearson correlation analysis and KEGG pathway analysis showed that the enhanced imaging strategy of aa-DESI/PI can facilitate the study of endogenous metabolic pathways and molecular networks. Moreover, the imaging results of mouse tumor tissue demonstrated the promising application of aa-DESI/PI in tumor research.