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BIBTEX RIS

Recovery of Brucella in raw milk Minas artisanal cheese approved for consumption by official inspection agency in Brazil: assessment of prevalence and risk factors through One Health integrated approaches

2022; Oxford University Press; Volume: 116; Issue: 11 Linguagem: Inglês

10.1093/trstmh/trac083

1878-3503

Márcio Roberto Silva, André Almeida Santos Duch, Rômulo Tadeu Pace de Assis Lage, Letícia Scafutto de Faria, Liliane Denize Miranda Menezes, João Batista Ribeiro, Guilherme Nunes de Souza, Paulo Martins Soares Filho, Ingred Sales Preis, Érica Bravo Sales, Patrícia Gomes de Souza, Flábio R. Araújo, Ricardo José de Paula Souza e Guimarães, Tulio Mendes, Christina Pettan-Brewer, Antônio Augusto Fonseca-Júnior,

Animal Disease Management and Epidemiology

Abstract Background Minas artisanal cheese (MAC) from the Serro region is a Brazilian intangible cultural heritage. Produced from raw milk, it may carry zoonotic pathogens such as Brucella. This study included a randomized survey for the prevalence of Brucella-positive MAC and its associated factors. Methods MAC samples (n=55), each one from a different rural family-based cheese-processing agroindustry, were analysed for Brucella by direct polymerase chain reaction (PCR) species-specific DNA detection and cultivation-based approaches. Results Among 55 MACs that were analysed, we found 17 Brucella DNA-positive samples (30.9% [95% confidence interval {CI} 18.7 to 43.1]) by PCR and, for the first time, from one MAC (1.8% [95% CI 0.5 to 9.7]), viable Brucella abortus was recovered by cultivation. Higher values for two variables, the number of lactating cows per herd (p=0.043) and daily milk production per herd (p=0.043), were each associated with Brucella-positive MAC, which concentrated in three high-risk and one low-risk spatial clusters. Conclusions MAC may be a source of Brucella for humans, since the positive samples were from batches that were sold by cheesemakers. This should be of concern and encourage cooperation between the health and agriculture sectors in order to mitigate this public health risk through One Health integrated approaches.