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Dual-color aggregation-induced emission nanoparticles for simultaneous lateral flow immunoassay of nitrofuran metabolites in aquatic products

2022; Elsevier BV; Volume: 402; Linguagem: Inglês

10.1016/j.foodchem.2022.134235

1873-7072

Xiuying Liu, Fei Xia, Shaoen Zhang, Yuanyuan Cheng, Ling Fan, Si Won Kang, Xue Gao, Xiaofei Sun, Jianrong Li, Xuepeng Li, Lijie Zhu,

Pharmacological Effects and Assays

Nitrofurans such as furaltadone and nitrofurantoin are a type of synthetic broad-spectrum antibiotics. Various fluorescent nanomaterials have been used as labeling materials in immunochromatographic assays (ICAs) for nitrofurans detection. However, previous fluorescent nanomaterials can undergo aggregation-caused quenching, leading to a decrease in the detection sensitivity. In this study, we developed a multiplex immunochromatographic assay (mICA) based on dual-color aggregation-induced emission nanoparticles (AIENPs) as signal labels for the simultaneous detection of 3-amino-5-morpholino-methyl-1,3-oxazolidinone (AMOZ) and 1-aminohydantoin (AHD), which were the metabolites of furaltadone and nitrofurantoin, respectively. Under optimal conditions, the cut-off values of the mICA for derivatized AMOZ (2-NP-AMOZ) and AHD (2-NP-AHD) reached up to 3 and 5 ng/mL, respectively. These values are at least 166-and 200-fold higher than those of the commercial gold nanoparticles (GNPs)-based test strip, respectively. Furthermore, the test strip was successfully applied to the samples, with acceptable recoveries in the range of 83.0-98.2%.