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New Pregnane Glycosides from Mandevilla dardanoi and Their Anti-Inflammatory Activity

2022; Multidisciplinary Digital Publishing Institute; Volume: 27; Issue: 18 Linguagem: Inglês

10.3390/molecules27185992

1433-1373

Francisca Sabrina Vieira Lins, Thalisson Amorim de Souza, Luı́za Carolina França Opretzka, Joanda P. R. e Silva, Laiane C. O. Pereira, Lucas Silva Abreu, Anderson Angel Vieira Pinheiro, George Luís Dias dos Santos, Yuri Mangueira do Nascimento, José Iranildo Miranda de Melo, Raimundo Braz‐Filho, Cristiane Flora Villarreal, Marcelo Sobral da Silva, Josean Fechine Tavares,

Phytochemistry and Biological Activities

Mandevilla Lindl. is an important genus of the Apocynaceae family, not only as ornamental plants but also for its medicinal uses. In Brazil, Mandevilla species are indicated to treat asthma and skin infections, their anti-inflammatory potential and wound healing properties are also reported in the literature. Concerning their chemical composition, this group of plants is a conspicuous producer of pregnane glycosides. Mandevilla dardanoi is an endemic species from the Brazilian semiarid region not studied by any phytochemical methods. In view of the medicinal potential of Mandevilla species, this study aimed to isolate new pregnane glycosides from M. dardanoi. To achieve this main goal, modern chromatography techniques were employed. Five new pregnane glycosides, dardanols A-E, were isolated from the roots of M. dardanoi by HPLC. Their structures were determined using extensive 1D and 2D-NMR and mass spectrometry (MSn and HRESIMS) data. The cytotoxicity and the anti-inflammatory potential of these compounds were evaluated. The first was evaluated by measuring proinflammatory cytokines and nitric oxide production by stimulated macrophages. Dardanols were able to inhibit the production of nitric oxide and reduce IL-1β and TNF-α. The current work demonstrates the chemodiversity of Brazilian semiarid species and contributes to amplifying knowledge about the biological potential of the Mandevilla genus.