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Genomic Variability of Hepatitis B Virus Circulating in Brazilian Western Amazon

2022; Multidisciplinary Digital Publishing Institute; Volume: 14; Issue: 10 Linguagem: Inglês

10.3390/v14102100

1999-4915

Tárcio Peixoto Roca, Lívia Melo Villar, Felipe Souza Nogueira Lima, Mariana Pinheiro Alves Vasconcelos, Lourdes Maria Pinheiro Borzacov, Eugênia de Castro e Silva, Bárbara V. Lago, Mayara Torquato Lima Silva, Luan Felipo Botelho Souza, Juan Miguel Villalobos Salcêdo, Alcione de Oliveira dos Santos, Deusilene Souza Vieira,

HIV Research and Treatment

The emergence of clinically relevant mutations in the hepatitis B virus (HBV) genome has been a matter of great debate because of the possibility of escape from the host’s immune system, the potential to cause more severe progression of liver diseases and the emergence of treatment-resistant variants. Here we characterized the circulating variants of HBV in Rondônia State, in the north of Brazil. Serum samples of 62 chronic HBV carriers were subjected to PCR assays and clinical data were collected. Mutations and genotypes were characterized through direct sequencing. The findings show the presence of subgenotypes A1 (54.83%, 34/62), D3 (16.13%, 10/62), F2 (16.13%, 10/62), A2 (4.84%, 3/62), D2 (3.23%, 2/62), D1 (1.61%, 1/62), D4 (1.61%, 1/62) and F4 (1.61%, 1/62). Deletions in the pre-S2 region were found in 13.79% (8/58) of the samples, mutations in the S gene in 59.68% (37/62) and RT mutations in 48.39% (30/62). We found a variable genotypic distribution in different locations and important mutations related to immune escape and drug resistance in Western Amazonia, which contributed to genetic surveillance and provided important information to help control the disease.