Produção Nacional
Revisado por pares
Activity of arginine-phenylalanine and arginine-tryptophan-based surfactants against Staphylococcus aureus
2022; Future Medicine; Volume: 17; Issue: 17 Linguagem: Inglês
10.2217/fmb-2022-0074
ISSN1746-0921
AutoresLetícia Serpa Sampaio Moreno, Francisca Bsa Nascimento, Cecília Rocha da Silva, Lívia Gurgel do Amaral Valente Sá, João B A de Neto, Jacilene Silva, Emmanuel M Silva, Hélcio Silva dos Santos, Lourdes Pérez, A. R. Da Silva, Daniel Sampaio Rodrigues, Amanda Dias Barbosa, Lara Elloyse Almeida Moreira, Bruno C. Cavalcanti, Manoel Odorico de Morais, Hélio Vitoriano Nobre Júnior,
Tópico(s)Biopolymer Synthesis and Applications
ResumoAims: This study aimed to evaluate the antibacterial effect of two new cationic surfactants based on phenylalanine-arginine (LPAM) and tryptophan-arginine (LTAM). Materials & methods: Antibacterial activity, mechanism of action and interactions with Staphylococcus aureus enzymes were measured through microbiological, flow cytometry and molecular docking assays, respectively. Results & conclusion: These compounds showed antibacterial activity in the range of 4.06–16.24 μg/ml against planktonic cells and no activity against mature biofilms, since they caused a loss of membrane integrity and increased DNA damage, as revealed by flow cytometry analysis. In silico assays revealed the existence of molecular bonds such as hydrogen bonds, mainly with DNA. Therefore, these compounds have promising pharmacological activity against MRSA strains.
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