ABSENCE OF EXPRESSION OF TRANSFORMING GROWTH FACTOR-beta TYPE II RECEPTOR IS ASSOCIATED WITH AN AGGRESSIVE GROWTH PATTERN IN A MURINE RENAL CARCINOMA CELL LINE, RENCA
1998; Lippincott Williams & Wilkins; Volume: 160; Issue: 5 Linguagem: Inglês
10.1016/s0022-5347(01)62437-6
ISSN1527-3792
AutoresShilajit Kundu, Isaac Yi Kim, David J. Zelner, Lynn Janulis, Shannon Goodwin, Jason D. Engel, CHUNG LEE,
Tópico(s)Renal and related cancers
ResumoNo AccessJournal of UrologyInvestigative Urology1 Nov 1998ABSENCE OF EXPRESSION OF TRANSFORMING GROWTH FACTOR-beta TYPE II RECEPTOR IS ASSOCIATED WITH AN AGGRESSIVE GROWTH PATTERN IN A MURINE RENAL CARCINOMA CELL LINE, RENCA SHILAJIT D. KUNDU, ISAAC Y. KIM, DAVID ZELNER, LYNN JANULIS, SHANNON GOODWIN, JASON D. ENGEL, and CHUNG LEE SHILAJIT D. KUNDUSHILAJIT D. KUNDU , ISAAC Y. KIMISAAC Y. KIM , DAVID ZELNERDAVID ZELNER , LYNN JANULISLYNN JANULIS , SHANNON GOODWINSHANNON GOODWIN , JASON D. ENGELJASON D. ENGEL , and CHUNG LEECHUNG LEE View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)62437-6AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Transforming growth factor-beta 1 (TGF-beta 1) inhibits the proliferation of many cancer cells. However, tumor cells frequently become resistant to this inhibitory effect due to the absence of TGF-beta receptor (T beta R) expression. This study reports the nature of TGF-beta sensitivity in an aggressive murine renal carcinoma cell line, Renca, investigated in a series of experiments. The growth of Renca cells, in tissue culture, was not sensitive to the inhibitory effect of TGF-beta 1 with doses ranging from 0.1 to 10 ng./ml., nor was this cell line sensitive to the effect of TGF-beta 1 in inducing the expression of plasminogen activator inhibitor-I. Renca cells expressed TGF-beta 1 mRNA and protein, as determined by RT-PCR and ELISA, respectively. The level of TGF-beta 1 production by Renca cells was moderate, thus eliminating the possibility that endogenous TGF-beta 1 production might be masking the effect of TGF-beta sensitivity. Furthermore, Renca cells expressed T beta R-I mRNA, but did not express T beta R-II mRNA, suggesting that the absence of this receptor may be the cause of TGF-beta insensitivity. Additionally, a vector containing the T beta R-II cDNA was transiently transfected into Renca cells. 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Volume 160Issue 5November 1998Page: 1883-1888 Advertisement Copyright & Permissions© 1998 by American Urological Association, Inc.MetricsAuthor Information SHILAJIT D. KUNDU More articles by this author ISAAC Y. KIM More articles by this author DAVID ZELNER More articles by this author LYNN JANULIS More articles by this author SHANNON GOODWIN More articles by this author JASON D. ENGEL More articles by this author CHUNG LEE More articles by this author Expand All Advertisement PDF downloadLoading ...
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