Artigo Acesso aberto

In vitro Regeneration of Sugarcane (Saccharum officinarum) Varieties GUI 11 and PMA 96/48

2018; Volume: 7; Issue: 1 Linguagem: Inglês

10.31254/jsir.2018.7102

ISSN

2320-4818

Autores

Kay Thi OO, Maung Maung Htwe, Nwe Ni San,

Tópico(s)

Sugarcane Cultivation and Processing

Resumo

The present investigation is deals with in vitro regeneration of two sugarcane varieties from Yezin, Pyinmana, Myanmar. The young suckers of two sugarcane varieties GUI11 and PMA 96/48 were used as explants. In this research, both sugarcane varieties gave high shoot proliferation rate on MS medium plus hormone BAP and NAA combinations. The shoot regeneration results of both varieties were not significant on MS basal medium supplemented with BAP + IBA (1+0.2, 1+0.5, 1+0.7, 0.5+0.1, 0.5+0.2, 0.5+ 0.3 mg/l). The maximum shoot formation was observed in MS medium supplemented with BAP 1mg/l + IBA 0.2 mg/l for both GUI 11 and PMA 96/48. The rooting experiment was conducted in half strength MS medium with various concentrations of NAA. The root formation from regenerated shoot was observed in half strength of MS medium supplemented with NAA 4, 5, 6 mg/l for both sugarcane varieties. Among three levels of NAA tested, the highest root formation was found at 5mg/l in GUI 11 and 6mg/l in PMA 96/48.

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