Microbiologic Evaluation of a Hydrogen Peroxide Sterilization System
1994; Volume: 25; Issue: 10 Linguagem: Inglês
10.3928/1542-8877-19941101-07
ISSN2325-8179
AutoresD L Wilkins, Paul Y Chung, Pam Y Tsuchiya, Izak F Wessels, Anthony J Zuccarelli,
Tópico(s)3D Printing in Biomedical Research
ResumoABSTRACT The reliability of chemical sterilizers (acetone and/or 30-percent hydrogen peroxide at 25° C and at 60° C) was tested against Bacillus subtilis inoculated onto glass slides, commercial biological indicator discs (Bacillus stearothermophilus and B. subtilis], and B. subtilis spore survival. Acetone alone was not sporicidal. Hydrogen-peroxide-sterilized glass slides were sterile after 5 minutes. The indicator discs required 25 minutes at 25" C, and less than 3 minutes at 60° C (P < .0001). The D value of B. subtilis in 27-percent hydrogen peroxide at 25° C is 2 minutes, with z values of 22° C and 26° C at 25° C and 40° C, respectively. For delicate instruments, a 30-percent peroxide solution followed by an acetone rinse provides an effective alternative to classic heat sterilization.
Referência(s)