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The Catalan Surveillance Network of SARS-CoV-2 in Sewage: design, implementation, and performance

2022; Nature Portfolio; Volume: 12; Issue: 1 Linguagem: Inglês

10.1038/s41598-022-20957-3

2045-2322

Laura Guerrero-Latorre, Neus Collado, Nerea Abasolo-Zabalo, Gabriel Anzaldi-Varas, Sílvia Bofill-Mas, Albert Bosch, Lluís Bosch, Sı́lvia Busquets, Antoni Caimari, Núria Canela-Canela, Albert Carcereny, Carme Chacón, Pilar Ciruela, Irene Corbella, Xavier Domingo, Xavier Escoté, Yaimara Espiñeira, Eva Forés, Isabel Gandullo-Sarró, David García-Pedemonte, Rosina Gironés, Susana Guix, Ayalkibet Hundesa, Marta Itarte, Roger Mariné-Casadó, Anna Martínez, Sandra Martínez‐Puchol, Anna Mas‐Capdevila, Cristina Mejías-Molina, Marc Moliner i Rafa, Antoni Munné, Rosa M Pintó, Josep Pueyo‐Ros, Jordi Robusté-Cartró, Marta Rusiñol, Robert Sanfeliu, Joan Teichenne-Jané, Helena Torrell-Galceran, Lluís Corominas, Carles Borrego,

Biosensors and Analytical Detection

Abstract Wastewater-based epidemiology has shown to be an efficient tool to track the circulation of SARS-CoV-2 in communities assisted by wastewater treatment plants (WWTPs). The challenge comes when this approach is employed to help Health authorities in their decision-making. Here, we describe the roadmap for the design and deployment of SARSAIGUA, the Catalan Surveillance Network of SARS-CoV-2 in Sewage. The network monitors, weekly or biweekly, 56 WWTPs evenly distributed across the territory and serving 6 M inhabitants (80% of the Catalan population). Each week, samples from 45 WWTPs are collected, analyzed, results reported to Health authorities, and finally published within less than 72 h in an online dashboard ( https://sarsaigua.icra.cat ). After 20 months of monitoring (July 20–March 22), the standardized viral load (gene copies/day) in all the WWTPs monitored fairly matched the cumulative number of COVID-19 cases along the successive pandemic waves, showing a good fit with the diagnosed cases in the served municipalities (Spearman Rho = 0.69). Here we describe the roadmap of the design and deployment of SARSAIGUA while providing several open-access tools for the management and visualization of the surveillance data.