First Report of ‘ Candidatus Phytoplasma Palmae’ (16SrIV-A Subgroup) Associated with Palm Lethal Yellowing Disease on Cocos nucifera and Pritchardia sp. in Guadeloupe, French West Indies
2022; American Phytopathological Society; Volume: 107; Issue: 5 Linguagem: Inglês
10.1094/pdis-08-22-1898-pdn
ISSN1943-7692
AutoresFabian Pilet, Marianne Loiseau, C. Boyer, Aurore Cavalier, C. Diman,
Tópico(s)Plant Pathogenic Bacteria Studies
ResumoHomePlant DiseaseVol. 107, No. 5First Report of ‘Candidatus Phytoplasma Palmae’ (16SrIV-A Subgroup) Associated with Palm Lethal Yellowing Disease on Cocos nucifera and Pritchardia sp. in Guadeloupe, French West Indies PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of ‘Candidatus Phytoplasma Palmae’ (16SrIV-A Subgroup) Associated with Palm Lethal Yellowing Disease on Cocos nucifera and Pritchardia sp. in Guadeloupe, French West IndiesF. Pilet, M. Loiseau, C. Boyer, A. Cavalier, and C. DimanF. Pilet†Corresponding author: F. Pilet; E-mail Address: [email protected]https://orcid.org/0000-0003-0079-216XCIRAD, UMR PVBMT, F-97410 Saint-Pierre, La Réunion, FranceSearch for more papers by this author, M. LoiseauAnses, National Plant Health Laboratory, Bacteriology, Virology and GMO Unit, Angers, FranceSearch for more papers by this author, C. BoyerCIRAD, UMR PVBMT, F-97410 Saint-Pierre, La Réunion, FranceSearch for more papers by this author, A. CavalierFredon, F-97130 Capesterre-Belle-Eau, Guadeloupe, FranceSearch for more papers by this author, and C. DimanDAAF, F-97108 Basse-Terre, Guadeloupe, FranceSearch for more papers by this authorAffiliationsAuthors and Affiliations F. Pilet1 † M. Loiseau2 C. Boyer1 A. Cavalier3 C. Diman4 1CIRAD, UMR PVBMT, F-97410 Saint-Pierre, La Réunion, France 2Anses, National Plant Health Laboratory, Bacteriology, Virology and GMO Unit, Angers, France 3Fredon, F-97130 Capesterre-Belle-Eau, Guadeloupe, France 4DAAF, F-97108 Basse-Terre, Guadeloupe, France Published Online:17 May 2023https://doi.org/10.1094/PDIS-08-22-1898-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleLethal yellowing (LY) disease causes major damage to palms in Central America and the Caribbean. It has been reported as far south as Antigua (Myrie et al. 2014). LY affects over 40 palm species, seriously impacts the coconut industry, and alters the landscapes on islands with a tourist-based economy. In March 2021, the presence of LY disease was regularly monitored in Guadeloupe. Two palm species (Cocos nucifera and Pritchardia sp.) died on a private property in Sainte-Anne, Grande Terre. Yellowing of lower fronds and necrosis of inflorescences were reported on some neighboring palms. One symptomatic C. nucifera (GP21-007) and four symptomatic Pritchardia sp. (GP21-005, GP21-006, GP21-008, and GP21-009) were sampled by stem drilling. Samples from four asymptomatic coconut trees (GP21-001 to GP21-004) were collected in the locality of Deshaies. DNA was extracted from the nine sawdust samples following a cetyltrimethylammonium bromide modified protocol (Doyle and Doyle 1990). A quantitative polymerase chain reaction (PCR), following the protocol described by Christensen et al. (2004), was performed on DNA to diagnose the presence of phytoplasmas. An exponential amplification was observed for all DNA extracts from symptomatic palm samples (threshold number of PCR cycles (Ct) ranged from 18.50 to 23.58). DNA from asymptomatic samples yielded negative results (undetermined Ct). To identify the phytoplasma associated with LY, DNA samples were subjected to PCR, based on the 16SrRNA gene, plus internal transcribed spacers (ITS) using P1-1T (Pilet et al. 2021)/P7 (Schneider et al. 1995) primers, and secA gene using the primer pair secAFor1/secARev1 (Hodgetts et al. 2008). Amplicons of 1.8 kb covering the 16S ribosomal operon and 830 bp for the secA gene were produced using DNA from symptomatic trees. All amplicons were double strand sequenced (Genewiz, U.K.). The corresponding sequences were deposited in GenBank and subjected to BLASTn on NCBI. Sequences of the ribosomal operon gene (accession nos. ON521114 to ON521118) were identical for the five positive samples. Sequencing revealed two distinct ribosomal operons with heterozygous peaks on the DNA chromatogram. The first amino ambiguity (M = adenine or cytosine) was observed in the 16Sr RNA gene. The second was observed in the first intergenic transcript spacer. The 16S rDNA sequence (M = cytosine) presented 100% identity with accession number HQ613874 and 99.93% with accession number U18747, the reference sequence for ‘Candidatus Phytoplasma palmae’. The virtual restriction fragment length polymorphism pattern derived from the 16S rDNA F2nR2 fragment and identified using iPhyclassifier (Zhao et al. 2009) was identical to the reference pattern for the 16SrIV-A subgroup. A unique sequence was obtained for the partial secA gene (OP136139 to OP136143), sharing 100% identity with EU267187 for the palm LY phytoplasma preprotein translocase subunit (secA) gene. This is the first report of ‘Ca. P. palmae’ (subgroup 16SrIV-A) associated with palm LY disease on C. nucifera and Pritchardia sp. in Guadeloupe. Measures to eradicate LY were implemented as soon as its presence was confirmed in Guadeloupe. LY phytoplasmas continue to spread in the Caribbean and are approaching South America, where the known vector, Haplaxius crudus, has already been reported (Silva et al. 2019). This poses a major threat to the coconut economy and the diversity of palm trees.The author(s) declare no conflict of interest.References:Christensen, N. M., et al. 2004. Mol. Plant-Microbe. Interact. 17:1175. https://doi.org/10.1094/MPMI.2004.17.11.1175 Link, ISI, Google ScholarDoyle, J. J., and Doyle, J. L. 1990. Focus 12:13. Google ScholarHodgetts, J., et al. 2008. Int. J. Syst. Evol. Microbiol. 58:1826. https://doi.org/10.1099/ijs.0.65668-0 Crossref, ISI, Google ScholarMyrie, W. A., et al. 2014. New Dis. 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Microbiol. 59:2582. https://doi.org/10.1099/ijs.0.010249-0 Crossref, ISI, Google ScholarThe author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 107, No. 5 May 2023SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 31 May 2023Published: 17 May 2023First Look: 20 Oct 2022Accepted: 16 Oct 2022 Page: 1621 Information© 2023 The American Phytopathological SocietyKeywordsCocos nuciferaGuadeloupelethal yellowingpalmphytoplasmaPritchardia sp.The author(s) declare no conflict of interest.PDF download
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