First Report of Botryosphaeria dothidea Causing Leaf Spot of Camellia oleifera in China
2022; American Phytopathological Society; Volume: 107; Issue: 5 Linguagem: Inglês
10.1094/pdis-06-22-1452-pdn
ISSN1943-7692
AutoresYi Hao, Kang Liao, Jianrong Guo, Chenxin Jin, Kaifa Guo, Ming Chen,
Tópico(s)Yeasts and Rust Fungi Studies
ResumoHomePlant DiseaseVol. 107, No. 5First Report of Botryosphaeria dothidea Causing Leaf Spot of Camellia oleifera in China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Botryosphaeria dothidea Causing Leaf Spot of Camellia oleifera in ChinaY. L. Hao, K. Liao, J. Guo, C. Z. Jin, K. F. Guo, and M. ChenY. L. HaoHunan University of Humanities, Science and Technology/Collaborative Innovation Center for Farmland Weeds Control, Loudi 417000, China, K. LiaoHunan University of Humanities, Science and Technology/Collaborative Innovation Center for Farmland Weeds Control, Loudi 417000, China, J. GuoHunan University of Humanities, Science and Technology/Collaborative Innovation Center for Farmland Weeds Control, Loudi 417000, China, C. Z. JinHunan University of Humanities, Science and Technology/Collaborative Innovation Center for Farmland Weeds Control, Loudi 417000, China, K. F. Guo†Corresponding author: K. F. Guo; E-mail Address: [email protected]https://orcid.org/0000-0002-8935-3923Hunan University of Humanities, Science and Technology/Collaborative Innovation Center for Farmland Weeds Control, Loudi 417000, China, and M. ChenHunan University of Humanities, Science and Technology/Collaborative Innovation Center for Farmland Weeds Control, Loudi 417000, ChinaAffiliationsAuthors and Affiliations Y. L. Hao K. Liao J. Guo C. Z. Jin K. F. Guo † M. Chen Hunan University of Humanities, Science and Technology/Collaborative Innovation Center for Farmland Weeds Control, Loudi 417000, China Published Online:17 May 2023https://doi.org/10.1094/PDIS-06-22-1452-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleCamellia oleifera Abel., a small evergreen tree or shrub, is mainly distributed in central and southern China with a larger scale of 4.5 × 106 ha (Zhu 2020). In May 2021, severe leaf spots were observed in plantation located in Shuangfeng County (27.4136°N, 111.5660°E), Hunan Province, China. More than 60 C. oleifera plants were surveyed with over 80% disease incidence. The symptoms on leaves were initially small brown lesions from leaf margins or tips, developing to suborbicular or irregular-shaped dark brown lesions, leading to leaves withering. A total of 60 symptomatic samples were randomly collected. Lesion margins were surface sterilized in 2% sodium hypochlorite for 1 min, rinsed with sterile distilled water for three times, dried, placed on potato dextrose agar (PDA), and incubated at 25°C in the dark for 3 days. Hyphal sections from colony edges were transferred to new PDA plates. Three isolates of Botryosphaeria dothidea were obtained. Colonies of B. dothidea were initially white, gradually turning dark gray with dense aerial mycelium after 6 days. To induce sporulation, colonies of YCB17 were transferred to synthetic nutrient-poor agar with sterilized leaves of C. oleifera. Cultures were initially incubated at 25°C in the dark for 3 days and were then alternatively exposed to 12-h near-UV light and 12-h white light (Chu et al. 2021). After 5 days, conidia that formed on leaves were examined microscopically. The conidia were unicellular, aseptate, hyaline, and fusoid, 20.9 to 25.5 × 4.7 to 6.4 μm (n = 50). Morphological characteristics of the isolates matched the description of B. dothidea (Slippers et al. 2014). The DNA sequence was amplified using primer pairs ITS1/ITS4 (Tang et al. 2022), EF1-728F/986R (Slippers et al. 2004), and βt2a/2b (Glass and Donaldson 1995), respectively. The sequences of three isolates (YCB2, YCB3, and YCB17) were deposited in GenBank with accession numbers ON714603, MZ613350, MZ613349 (ITS), OM328342, OM328343, OM328344 (TEF-1α), and OM328345, OM328346, OM328347 (TUB2). A BLAST search of sequences showed the ITS, TEF-1α, and TUB2 sequences had >99% identity with homologous sequences from B. dothidea isolates IRNHM-KZ49 (MG198191.1), CAP288 (EF638732.1), and Mu1 (MK423987.1), respectively. For pathogenicity testing, healthy leaves of 2-year-old C. oleifera plants in the greenhouse were spray-inoculated with conidial suspension (2 × 106 conidia/ml) from YCB17. Ten surface-sterilized and wounded leaves per plant were sprayed with 30 μl of suspension. Another 10 wounded leaves sprayed with sterile distilled water served as a control. All plants were kept in the greenhouse with temperature at 26 ± 2°C and 50% relative humidity. After 12 days, initial symptoms were observed on more than 80% of leaves inoculated with conidial suspension, whereas no symptoms were observed on the control leaves. The test was repeated three times with similar results. It was found that B. dothidea could cause leaf spot of C. oleifera. The infected leaves showed the same symptoms as the samples. Reisolated fungi from infected leaves were morphologically identical to B. dothidea. B. dothidea has been reported causing leaf spot in a wide range of hosts but has not previously been reported causing disease on C. oleifera. To our knowledge, this is the first report of B. dothidea causing leaf spot of C. oleifera in China. The information on identification of this fungus may be helpful to the control and prevention of the disease.The author(s) declare no conflict of interest.References:Chu, R.-T., et al. 2021. Mycosystema 40:473. Google ScholarGlass, N. L., and Donaldson, G. C. 1995. Appl. Environ. Microbiol. 61:1323. https://doi.org/10.1128/AEM.61.4.1323-1330.1995 Crossref, ISI, Google ScholarSlippers, B., et al. 2004. Mycologia 96:83. https://doi.org/10.1080/15572536.2005.11833000 Crossref, ISI, Google ScholarSlippers, B., et al. 2014. 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X. 2020. http://gz.people.com.cn/n2/2020/1119/c194844-34425098.html Google ScholarFunding: Funding was provided by the Special Science Popularization Topic for the Construction of Innovative Provinces (2021ZK4440).The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 107, No. 5 May 2023SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 31 May 2023Published: 17 May 2023First Look: 25 Oct 2022Accepted: 24 Oct 2022 Page: 1632 Information© 2023 The American Phytopathological SocietyFundingSpecial Science Popularization Topic for the Construction of Innovative ProvincesGrant/Award Number: 2021ZK4440KeywordsBotryosphaeria dothideaCamellia oleiferaleaf spotThe author(s) declare no conflict of interest.PDF downloadCited byFirst Report of Trunk Canker Caused by Botryosphaeria dothidea on Cinnamomum camphora in ChinaXiaoxiao Feng, Lu Liu, Ying Jin, Xiaoni Yan, Peiqi Zhongzhu, Guorong Wang, Chunying Qiu, and Huiming Wu17 November 2023 | Plant Disease, Vol. 0, No. 0First Report of Botryosphaeria dothidea Causing Brown Leaf Spot on Daimyo Oak (Quercus dentata) in ChinaW. 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