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Gallic acid has an inhibitory effect on skin squamous cell carcinoma and acts on the heat shock protein HSP90AB1

2022; Elsevier BV; Volume: 851; Linguagem: Inglês

10.1016/j.gene.2022.147041

1879-0038

Sabrina Ferreira de Jesus, Marcela Gonçalves de Souza, Lorena dos Reis Pereira Queiroz, Daniela Paola Santos de Paula, Angeliny Tamiarana Lima Tabosa, Wislene Sarajane Moreira Alves, Luiz Henrique da Silveira, André Teixeira‐Ferreira, Ozires José Dutra Martuscelli, Lucyana Conceição Farias, Alfredo Maurício Batista de Paula, Sérgio Henrique Sousa Santos, André Luiz Sena Guimarães,

Heat shock proteins research

Differences in the features of aggressiveness of non-melanoma skin cancer (NMSC) subtypes, between basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) are relevant characteristics. Comparing the characteristics between NMSC subtypes might help identify molecules associated with cancer metastasis and invasion. Considering these facts, the current study aimed to identify a molecular target for inhibiting skin cancer metastasis and invasion. Proteomic analysis suggested that heat shock protein 90 kDa, alpha, class B member 1 (HSP90AB1), pentaxin (PTX3), caspase-14 (CASP14), S100, actin-1, and profilin were the primary targets related to metastasis and invasion. However, after a differential expression comparison between BCC and SCC, HSP90AB1 was identified as the best target to repress metastasis and invasion. Based on molecular docking results, gallic acid (GA) was selected to inhibit HSP90AB1. A specific Hsp90ab1 siRNA targeting was designed and compared to GA. Interestingly, GA was more efficient in silencing HSP90AB1 than siRNAhsp90ab1. Hence, our data suggest that HSP90AB1 is a crucial biomarker for identifying invasion and metastasis and that its inhibition may be a viable strategy for treating skin cancer.