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First Report of Myrtle Rust Caused by Austropuccinia psidii on Eugenia myrcianthes in Brazil
2022; American Phytopathological Society; Volume: 107; Issue: 7 Linguagem: Inglês
10.1094/pdis-07-22-1569-pdn
ISSN1943-7692
AutoresCaliandra Bernardi, Maristela dos Santos Rey, Américo Wagner Júnior, Nelson Bernardi Lima, Diogo Rovaris Biz, Viviane da Rosa,
Tópico(s)Plant Pathogens and Resistance
ResumoHomePlant DiseaseVol. 107, No. 7First Report of Myrtle Rust Caused by Austropuccinia psidii on Eugenia myrcianthes in Brazil PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Myrtle Rust Caused by Austropuccinia psidii on Eugenia myrcianthes in BrazilCaliandra Bernardi, Maristela Santos Rey, Américo Wagner Júnior, Nelson Bernardi Lima, Diogo Rovaris Biz, and Viviane da RosaCaliandra BernardiUniversidade Tecnológica Federal do Paraná, Laboratório de Fitopatologia 85660-000, Dois Vizinhos, PR, BrazilSearch for more papers by this author, Maristela Santos Rey†Corresponding author: M. S. Rey; E-mail Address: [email protected]https://orcid.org/0000-0002-9323-0395Universidade Tecnológica Federal do Paraná, Laboratório de Fitopatologia 85660-000, Dois Vizinhos, PR, BrazilSearch for more papers by this author, Américo Wagner JúniorUniversidade Tecnológica Federal do Paraná, Laboratório de Fitopatologia 85660-000, Dois Vizinhos, PR, BrazilSearch for more papers by this author, Nelson Bernardi Limahttps://orcid.org/0000-0002-6145-5119Universidad Nacional de Catamarca (UNCA), Cátedra de Fitopatología, C.P. 4700, Catamarca, ArgentinaSearch for more papers by this author, Diogo Rovaris BizUniversidade Tecnológica Federal do Paraná, Laboratório de Fitopatologia 85660-000, Dois Vizinhos, PR, BrazilSearch for more papers by this author, and Viviane da RosaUniversidade Tecnológica Federal do Paraná, Laboratório de Fitopatologia 85660-000, Dois Vizinhos, PR, BrazilSearch for more papers by this authorAffiliationsAuthors and Affiliations Caliandra Bernardi1 Maristela Santos Rey1 † Américo Wagner Júnior1 Nelson Bernardi Lima2 Diogo Rovaris Biz1 Viviane da Rosa1 1Universidade Tecnológica Federal do Paraná, Laboratório de Fitopatologia 85660-000, Dois Vizinhos, PR, Brazil 2Universidad Nacional de Catamarca (UNCA), Cátedra de Fitopatología, C.P. 4700, Catamarca, Argentina Published Online:16 Jun 2023https://doi.org/10.1094/PDIS-07-22-1569-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleThe Eugenia myrcianthes fruit can be consumed in natural or processed form (in jellies and juices) (Infante et al. 2016). In 2019, in the Universidade Tecnológica Federal do Paraná, Dois Vizinhos city, Paraná State, Brazil, yellow epiphyllous uredinias were observed on the tissue surface (leaves, stems, flowers, and fruit) of 21 trees of E. myrcianthes, which resembled structures typical of Myrtaceae rust. All colonized tissues showed necrotic lesions that varied in size and shape, causing death, especially in fruit. In the orchard, the fungus affected yield by 50 to 95%. Fruit (10) and leaves (20) with symptoms were collected from 11 trees from different positions in the orchard. Infected tissues were incubated (25°C and 12-h photoperiod) for 7 days to induce sporulation. The epiphyllous uredinia, united in small groups with hyaline and globose urediniospores, were observed and presented equinulate ornaments and germinated pores in the subequatorial and inordinate positions (Cummins and Hiratsuka 2003; De Pieri 2012) (mean 14.00 × 21.12 μm, n = 30) similar to the morphological characteristics of the Austropuccinia genus described by Young (2019). The identification of 10 samples (fruit and leaves) of the pathogen taken from infected parts of the trees was confirmed. For molecular identification, the internal transcribed spacer (ITS) region was amplified by polymerase chain reaction (White et al. 1990) and sequenced. Phylogenetic analyses using Bayesian inference grouped the isolate from E. myrcianthes with the epitype species of Austropuccinia psidii (Beenken 2017), with a high posterior probability (0.99). The sequences of one representative strain (Emg1) were submitted to GenBank (OM948983). For pathogenicity tests, three healthy branches containing 20 leaves were sprayed with 3.0 ml of urediniospore suspension (105) of Emg1 and covered with a plastic bag in the orchard (25°C). Sterile distilled water was used as a control. Three replications (pathogen and control) were performed on different trees. After 6 days, symptoms appeared, and their morphological features were similar to those previously reported. Control branches did not present fungal growth. The inoculation test was repeated again, confirming symptoms such as uredinia and urediniospores, characteristic of the disease. This is the first report of the incidence of A. psidii infection in E. myrcianthes trees in Brazil, causing rust, necrosis, and senescence in fruit, leaves, flowers, and stems. The rust on E. myrcianthes causes destructive damage to yield, as the pathogen causes fruit to rot and drop prematurely.The author(s) declare no conflict of interest.References:Beenken, L. 2017. Phytotaxa 297:53. https://doi.org/10.11646/phytotaxa.297.1.5 Crossref, ISI, Google ScholarCummins, G. B., and Hiratsuka, Y. 2003. Page 225 in: Illustrated Genera of Rust Fungi. American Phytopathological Society, St. Paul, MN. Google ScholarDe Pieri, C. 2012. Caracterização de Puccinia psidii, identificação de mirtáceas diferenciadoras de raças fisiológicas e estudos anatômicos do limbo foliar relacionados à resistência Universidade Estadual Paulista, São Paulo, Brazil. Google ScholarInfante, J., et al. 2016. PLoS One 11:01529744. https://doi.org/10.1371/journal.pone.0152974 Crossref, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Crossref, Google ScholarYoung, W. T. L. 2019. Plant Pathol. 68:547. https://doi.org/10.1111/ppa.12959 Crossref, ISI, Google ScholarFunding: Funding was provided by Conselho Nacional de Desenvolvimento Científico e Tecnológico.The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 107, No. 7 July 2023SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 26 Jul 2023Published: 16 Jun 2023First Look: 21 Nov 2022Accepted: 19 Nov 2022 Page: 2226 Information© 2023 The American Phytopathological SocietyFundingConselho Nacional de Desenvolvimento Científico e TecnológicoKeywordsfruitnativerustThe author(s) declare no conflict of interest.PDF download
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