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Royal jelly arranges apoptotic and oxidative stress pathways and reduces damage to liver tissues of rats by down-regulation of Bcl-2, GSK3 and NF-κB and up-regulation of caspase and Nrf-2 protein signalling pathways

2022; Taylor & Francis; Volume: 28; Issue: 2 Linguagem: Inglês

10.1080/1354750x.2022.2159526

1366-5804

Abdullah Nabi Aslan, Özlem Gök, Seda Beyaz, Gözde Parlak, Muhammed İsmail Can, Ramazan Gündoğdu, Serpil Başpinar, İbrahim Hanifi Özercan, Akif Evren Parlak,

Pharmacological Effects of Natural Compounds

AbstractIntroductionRoyal jelly (RJ) from the honey bee, Apis mellifera, is a traditional product that is widely used as a food supplement to support the medical treatment of various diseases.Material and methodsOur study continued for 8 weeks. 42 Wistar albino (8 weeks old) male rats were used in the study. The study included 6 groups; Group 1: Control group (fed with standard diet), Group 2: RJ (100 mg/kg, bw), Group 3: F-50 (50 mg/kg, bw), group 4: F-100 (100 mg/kg, bw) group 5: F-50 (50 mg/kg, bw) + RJ (100 mg/kg, bw) Group 6: F-100 (100 mg/kg, bw) + RJ (100 mg/kg, bw). Malondialdehyde (MDA), catalase (CAT) and glutathione (GSH) activities in liver tissue were determined by spectrophotometer. Liver tissue samples were examined histopathologically and various protein levels were determined by Western blotting technique.ResultsRJ caused a significant decrease in MDA level, Bcl-2, GSK3 and NF-κB protein expression levels, whereas induced a significant increase in GSH level, CAT activities and Bax, BDNF, caspase-6, caspase-3, Nrf-2 protein expression levels.ConclusionOur findings suggest RJ to be used as a hepatoprotective agent in the clinic to modulate the toxic effects of fluoride and other chemicals in the future.Keywords: Fluorideliver damageNF-κBNrf-2oxidative stressroyal jelly AcknowledgmentsSome of the results of this article were presented at the 9th International Molecular Biology and Biotechnology Congress (MOLBIOTECH) (6–10 December 2020 Kars, Turkey) and International European Conference on Interdisciplinary Scientific Researches-III (15–16 January 2021 Comrat, Moldova).Authors’ contributionsAA: wrote the artıcle, review-editing, investigation, methodology, formal analysis, OG: wrote the article, laboratory analysis, reading article, formal analysis SB: laboratory analysis, reading article, formal analysis, MIC: laboratory analysis, reading article, formal analysis, GP: laboratory analysis, reading article, IHO: histopathological analysis, formal analysis, RG: review-editing, SBP: laboratory analysis, AEP: laboratory analysisDisclosure statementNo potential conflict of interest was reported by the author(s)Ethical approvalBefore starting the study, the approval of Fırat University Animal Experiments Local Ethics Committee (20.08.2020 meeting date, 2020/11 meeting number) was obtained and this research was conducted in conformity with the Ethics Committee Directive.Informed consentAll authors approved publication of this article in Biomarkers.Data availability statementAll data of this study are included in this manuscript.Additional informationFundingThis study was supported by Firat University Research Projects Unit (FUBAP) [Project No: FF.19.16].