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A hatching aphidlion‐like lacewing larva in 100 million years old Kachin amber

2022; Wiley; Volume: 30; Issue: 3 Linguagem: Inglês

10.1111/1744-7917.13137

1744-7917

Joachim T. Haug, Kay Lwin Tun, Gideon T. Haug, Kyaw Naing Than, Carolin Haug, Marie K. Hörnig,

Plant and animal studies

We report a fossil aphidlion-like larva preserved with its egg case in 100 million year old Kachin amber, Myanmar. It appears to have been enclosed very shortly after hatching, especially when comparing it with extant aphidlions during hatching. Although hatching aphidlion-like larvae are known from amber from other localities, this is the first case from Myanmar amber, despite the comparably high number of lacewing larvae known from the latter. Dear Editor, Hatching is a crucial moment in the life of an animal. The animal stops being an embryo protected by the egg and becomes a post-embryonic immature (see Haug, 2020 for difficulties in naming these stages). This must have been the case in the past as well, but the fossil record is rather sparse in the preservation of this very moment. The fossil preservation of such a case needs to facilitate not only preservation of the animal, but also of the egg. As these are often of rather different material, making the preservation of both together in many cases unlikely. Yet, there are preservation types that seem to favor such incidents. Amber has become famous for preserving cases of "frozen behaviour", preserving a specific moment in the life of an animal in an almost life-like manner (Arillo, 2007), including: copulations, parasite-host interactions, aggregations, group defense, brood care, egg-laying (although likely stress-induced), but also animals hatching from their eggs (examples in e.g., Weitschat & Wichard, 2002; Engel & Grimaldi, 2008; Weitschat, 2009; Boucot & Poinar, 2010; Gröhn, 2015; Hörnig et al., 2016, 2019, 2020, 2022; Fischer & Hörnig, 2019; Pérez-de la Fuente et al., 2019). Preserved eggs are often difficult to identify as such in amber due to the low number of characters and often being rather soft. Also the identification of the "producer" of the egg can be challenging. This is different if organisms were captured right in the moment of hatching or during the process of egg laying, providing a link from a specific egg morphology to the corresponding animal. Known examples include assassin bugs hatching from their highly specialised eggs with distinct "lids", offering more characters to identify such eggs even as fossils (Hörnig et al., 2019), but also lacewings. A small lacewing larva in late Cretaceous Canadian amber (redrawn in Fig. 1B) is preserved in direct proximity to a stalked egg, well known, for example, in modern green lacewings (Chrysopidae; New, 1989 fig. 125-5 p. 87; redrawn in Fig. 1A), but also in some other groups of lacewings (Lucchese, 1956 fig. LV p. 179; redrawn in Fig. 1C). The specimen in amber was initially interpreted as a representative of Chrysopidae (Engel & Grimaldi, 2008 fig. 14 p. 58; redrawn in Fig. 1B), but later re-interpreted as a larva of beaded lacewings (Berothidae). In beaded lacewings, a clutch of eggs is attached to a single stalk (Brushwein, 1987 fig. 1 p. 674) or at least fewer stalks than there are eggs in the clutch (Toschi, 1964 fig. 2 p. 23), but there are also eggs with no stalk and a well developed surface structuring (Möller et al., 2006 fig. 1 p. 3; Monserrat, 2006 fig. 10a p. 194). Still, there are also species that produce individual eggs with a single stalk without strong surface sculpture, very similar to what is seen in the fossil (Tillyard, 1916 text-fig. 10 p. 320; Toschi, 1964 fig. 1 p. 22), supporting the interpretation as a beaded lacewing. More spectacular is a group of aphidlion-like larvae preserved in Cretaceous Lebanon amber (Pérez-de la Fuente et al., 2019; redrawn in Fig. 1D). No less than 13 larvae (some incomplete), with remains of eleven eggs have been reported, including egg bursters in some of the eggs, indicating that the event of hatching must have occurred just before the animals became embedded in the resin. As two examples of preserved hatching involve lacewing larvae, and one example includes a dozen specimens, it is partly surprising that we have so far not seen a similar case from Kachin amber, Myanmar. This type of amber has provided by far the majority of lacewing larvae, more than 200 specimens (Liu et al., 2016, 2018; Wang et al., 2016; Badano et al., 2018, 2021; Haug et al., 2019a–c, 2020a–c, 2021a–d, 2022a–d; Hörnig et al., 2020, 2022; Pérez-de la Fuente et al., 2020; Zippel et al., 2021; Haug & Haug, 2022; Liu et al., 2022). However, none of these included a case of hatching. We here report a new fossil aphidlion-like larva from Myanmar amber (Fig. 2A–E). It is preserved in close proximity to an empty egg case (Fig. 2A–C), indicating that it represents a newly hatched larva. The specimen is part of the Palaeo-Evo-Devo Research Group Collection of Arthropods at the Ludwig-Maximilians-Universität München, under repository number PED 1754. It was documented on a Keyence VHX-6000 digital microscope (for details, see Haug et al., 2019b). For comparison, eggs of modern green lacewings were collected on the campus of the University of Yangon, Yangon, Myanmar (Fig. 3A, B). Eggs were observed until the larvae hatched and were photographed/filmed with a Xiaomi Redmi Note 8 and under a stereo microscope (Olympus SZ 51) with an Apple iPhone 8 (Fig. 3C–L; in Supplementary Information Video S1). For detailed descriptions, see Supplementary Information. The mouth parts of the new specimen form prominent stylets without any teeth (Fig. 2D), and the trunk segments bear large protrusions (Fig. 2A–C; cf. MacLeod, 1964; Zimmermann et al., 2019). Very similar-appearing larvae have already been reported from many Cretaceous ambers from Spain (Pérez-de la Fuente et al., 2012, 2016), Lebanon (Pérez-de la Fuente et al., 2018, 2019), and Myanmar (Wang et al., 2016; Haug et al., 2022c). All these larvae have been interpreted as relatives of modern green lacewings (Chrysopidae), which are generally termed aphidlions. The fossils therefore represent aphidlion-like larvae; also the new fossil is apparently such an aphidlion-like lacewing larva. Wang et al. (2016) and Haug et al. (2022c) differentiated three morphotypes of aphidlion-like larvae in Myanmar amber with prominent protrusions, but the details partly differ. Based on the relative lengths of the protrusions, morphotype 1 seems to be similar in both cases. Liu et al. (2022) erected a new species, Acanthochrysa langae, based on a single aphidlion-like larvae. While generally falling into morphotype 1, Liu et al. (2022) pointed out differences that could be used to clearly diagnose the specimen from others: the more elongated head capsule and the longer stylets. Indeed this aspect seems to vary quite drastically among the aphidlion-like larvae in Myanmar (Haug et al., 2022c), indicating that there are several species represented within (at least some of) the morphotypes. The new specimen is best interpreted as morphotype 1, but differs from Acanthochrysa langae. Although not very well preserved, at the trunk end of the aphidlion-like larva an egg case is visible (Fig. 2A–C). It strongly resembles the egg cases reported by Pérez-de la Fuente et al. (2019). The egg case lacks signs of a stalk, similar to the egg cases from Pérez-de la Fuente et al. (2019). An egg burster is not preserved, but these were only preserved in very few egg cases reported in Pérez-de la Fuente et al. (2019); hence the absence in the new specimen does not appear unusual. Also the morphology of the larva is very similar to that of the larvae reported by Pérez-de la Fuente et al. (2019). As these were interpreted as having newly hatched from their eggs, this is also plausible for the new fossil. Most extant representatives of Chrysopidae are known to produce clusters of stalked eggs (Fig. 3B) from less than ten eggs, but usually about 200, reaching up to 600 (Hinton, 1981). It seems that the stalk of the eggs leads to a protection against predation and even cannibalism of conspecific larvae (Rùžička, 1997). The eggs are usually deposited close to putative prey of the larvae, especially close to aphid colonies, resulting in a better food availability for the freshly hatched aphidlions. The position close to aphids leads to a risk for the eggs, as aphids can be protected by ants. Hayashi and Nomura (2014) could show that the survival rate of eggs with intact stalks is distinctly higher compared to those with removed stalks in presence of ants, but not necessarily in presence of other predators (in this study Chrysoperla nipponensis). However, Gepp (1990) reported at least five different deposition forms of eggs within Chrysopidae. These include also unstalked eggs, either laid in loose groups or singly. The new specimen PED 1754 does not show any remains of an egg stalk. This lack could be a preservational artefact, or it means that a stalk was simply not present in this group of aphidlion-like representatives, as also known for some modern species of Chrysopidae. The strategy of stalked eggs was already present in lacewings in the Cretaceous as shown by the hatching beaded lacewing (Engel & Grimaldi, 2008), but not necessarily in Chrysopidae. Pérez-de la Fuente et al. (2019) assumed that, even if not preserved in the specimens, egg stalks might be present on eggs of Tragichrysa ovoruptora (Pérez-de la Fuente et al., 2019, fig. 5). While stalked eggs are the "typical" deposition type in modern representatives of Chrysopidae (Fig. 3), it does not seem to be part of the ground pattern. Therefore, a stalk might not necessarily have been present in Cretaceous representatives with aphidlion-like larvae, as a stalked egg may possibly have evolved later within the group. Hatching events for lacewing larvae have so far been reported from Canadian and Lebanon amber (Engel & Grimaldi, 2008; Pérez-de la Fuente et al., 2019). In contrast to many other insect groups, fossil findings of lacewings which allow to reconstruct aspects of behavior are comparatively common in the literature (camouflage, Engel & Grimaldi, 2007; Weitschat, 2009; Pérez-de la Fuente et al., 2012, 2016, 2018; Wang et al., 2016; digging behavior, Badano et al., 2018; group defense, Hörnig et al., 2022, different aspects of trophic interactions, Ohl, 2011; Haug et al., 2018; Hörnig et al., 2020; summarized in Haug et al., 2022). Most of these aspects of fossilized behavior in lacewing larvae have been reported from Myanmar amber (Haug et al., 2022), hence it was expectable that also a case of hatching should have been preserved. The new fossil finally fills this gap. The Volkswagen Foundation kindly supported the work of JTH in the frame of a Lichtenberg professorship. Part of the project was supported by the German Research Foundation under DFG Ha 6300/6-1. We thank all people providing low cost, open access and open source software. J. Matthias Starck, Munich, and Steffen Harzsch, Greifswald, are thanked for long-term support. This is LEON publication #46. Open Access funding enabled and organized by Projekt DEAL. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.