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BIBTEX RIS

Treatment of multiple sclerosis patients with interferon-β primes monocyte-derived macrophages for apoptotic cell death

2001; Oxford University Press; Volume: 70; Issue: 5 Linguagem: Inglês

10.1189/jlb.70.5.745

1938-3673

Johan Van Weyenbergh, Juana Wietzerbin, Dany Rouillard, Manoel Barral‐Netto, Roland Liblau,

Cell death mechanisms and regulation

Abstract Although interferon (IFN)-β has shown a significant clinical benefit in multiple sclerosis (MS), its mechanism of action remains unclear. We found that IFN-β treatment of patients with MS resulted in a significant increase in apoptotic cell death (measured by annexin V staining and nuclear fragmentation) of monocyte-derived macrophages, as compared with cells derived from patients before treatment. Stimulation of the cells with IFN-β in vitro resulted in an even further increase of annexin V binding, as well as increased Fas (CD 95, APO-1) expression. However, no increased Fas expression, apoptotic monocytes, or monocytopenia were observed upon in vivo treatment. This indicates that IFN-β does not deliver a death signal to monocytes but rather primes for subsequent macrophage apoptosis upon activation or differentiation.