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Phenotypic and genotypic characterization of the hemolysin enzyme produced by Escherichia coli isolated from different sources

2022; American Institute of Physics; Volume: 2398; Linguagem: Inglês

10.1063/5.0093763

1935-0465

Karrar Ali Hussein, Azhar Noory Hussein,

Antibiotic Resistance in Bacteria

The main purpose of this study was to isolation and identification of pathogenic E.coli, then detection their ability to produce hemolysin enzyme phenotypically, then determine the genetic location of gene which responsible for the production of this enzyme. So 300 specimens were collected from different sources distributed on (urine, stool, sputum, burns and wounds) of different age groups and for both sexes, from patients who reviewing and residents at Al-Hashemiyah, Imam Al-Sadiq and Children Hospitals in Hilla city during the period from 15/8/2020 to 15/1/2021, the results showed that from whole isolates, 60 isolates was E.coli.The ability of isolates to produce hemolysin enzyme was investigated through hemolysin plates containing four different human blood groups (A, B, AB, O), and it was found that 26 isolates (43%) were able to produce hemolysin enzyme on the hemolysin plates. for the blood groups (A, B, AB,O), the percentage of hemolysin production was (54%, 65%, 69%, 50%) respectively, as these isolates were used to study the phenotypic and genotypic characterization in relation to the production of hemolysin enzyme. from whole isolates no one was bearing the gene which encodes for hemolysin enzyme (hlyB gene) on plasmid DNA. The electrophoresis showed that only two bacterial isolates was containing (hlyB gene) on chromosomal DNA, a bundle for each isolate with a molecular weight of 512bp.