Produção Nacional
Revisado por pares
Evaluation of Genotoxicity and Toxicity of Annona muricata L. Seeds and In Silico Studies
2022; Multidisciplinary Digital Publishing Institute; Volume: 28; Issue: 1 Linguagem: Inglês
10.3390/molecules28010231
ISSN1433-1373
AutoresGleison Gonçalves Ferreira, Ana Carolina Sousa Quaresma, Dayse Lúcia do Nascimento Brandão, Andrey Moacir do Rosário Marinho, José Edson de Sousa Siqueira, Kamila Leal Correa, José Otávio Carréra Silva Júnior, Sandro Percário, Maria Fâni Dolabela,
Tópico(s)Phytochemical compounds biological activities
ResumoCancer is a multifactorial organic dysfunction for which great efforts are being devoted in searching for new treatments and therapeutic adjuvants. Annona muricata is a fruit that has promising activity against several types of cancer, as it contains acetogenins, the metabolite group associated with this action. Thus, the objective of this study was to evaluate, in experimental models, the toxic behavior of an extract and fraction rich in acetogenins from A. muricata seeds and study the acetogenin, Annonacin, in silico. Phytochemical characterization was made by thin layer chromatography, spectroscopy in the infrared region and nuclear magnetic resonance. Toxicity was evaluated by tests of Allium cepa and Artemia salina, and in silico studies using the SwissDock servers DockThor, PharmMapper, ADMETLab, PreADME, Osiris and ProTox. The extract and fraction showed genotoxic activity against meristematic cells of A. cepa, reducing the mitotic index; however, the extract produced great deleterious effects on the system, even causing cell necrosis. In A. Saline, the extract was more toxic than the fraction, but both samples were considered toxic. Annonacin was effectively linked to complex I, and presented different activities regarding toxicity. Thus, the results of this study are promising, highlighting the anticancer potential of acetogenins.
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