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BIBTEX RIS

MiRNA-30d and miR-770-5p as potential clinical risk predictors of Vasoplegic Syndrome in Patients undergoing on-pump coronary artery bypass grafting

2023; Nature Portfolio; Volume: 13; Issue: 1 Linguagem: Inglês

10.1038/s41598-023-28978-2

ISSN

2045-2322

Autores

Omar Asdrúbal Vilca Mejía, Renato Cesar de Souza, Aritânia Sousa Santos, Bianca Meneghini, Ana Carolina Carvalho Silva, Guilherme Visconde Brasil, Vagner Oliveira Carvalho Rigaud, Luís Roberto Palma Dallan, Luíz Felipe Pinho Moreira, Luiz Augusto Ferreira Lisboa, Luís Alberto Oliveira Dallan, Jorge Kalil, Edécio Cunha‐Neto, Ludmila Rodrigues Pinto Ferreira, Fábio Biscegli Jatene,

Tópico(s)

Cardiac and Coronary Surgery Techniques

Resumo

Abstract The aims of this study were to perform pre-surgery miRNA profiling of patients who develop Vasoplegic syndrome (VS) after coronary artery bypass grafting (CABG) and identify those miRNAs that could be used as VS prognostic tools and biomarkers. The levels of 754 microRNAs (miRNAs) were measured in whole blood samples from a cohort of patients collected right before the coronary artery bypass grafting (CABG) surgery. We compared the miRNA levels of those who developed VS (VASO group) with those who did not (NONVASO group) after surgery. Six miRNAs (hsa-miR-548c-3p, -199b-5p, -383-5p -571 -183-3p, -30d-5p) were increased and two (hsa-1236-3p, and hsa-miR770-5p) were decreased in blood of VASO compared to NONVASO groups. Receiver Operating Characteristic (ROC) curve analysis revealed that a combination of the miRNAs, hsa-miR-30d-5p and hsa-miR-770-5p can be used as VS predictors (AUC = 0.9615, p < 0.0001). The computational and functional analyses were performed to gain insights into the potential role of these dysregulated miRNAs in VS and have identified the “Apelin Liver Signaling Pathway” as the canonical pathway containing the most target genes regulated by these miRNAs. The expression of the combined miRNAs hsa-miR-30d and hsa-miR-770-5p allowed the ability to distinguish between patients who could and could not develop VS, representing a potential predictive biomarker of VS.

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