Intracellular expression of interleukin‐4 and interferon‐γ by a Mycobacterium tuberculosis antigen‐stimulated CD4 + CD57 + T‐cell subpopulation with memory phenotype in tuberculosis patients
2003; Wiley; Volume: 111; Issue: 1 Linguagem: Inglês
10.1111/j.1365-2567.2004.01785.x
ISSN1365-2567
AutoresMaría del Carmen Jiménez Martínez, M. Linares, Renata Báez, Luis F. Montaño, S Martínez-Cairo, Patrícia Gorocica, Raúl Chávez, Edgar Zenteno, Ricardo Lascuraín,
Tópico(s)T-cell and B-cell Immunology
ResumoSummary In some chronic pathological conditions, antigen persistence activates and expands the CD4 + CD57 + T‐cell subset. The host immune response against tuberculosis infection is maintained through the continuous presence of antigen‐stimulated effector/memory helper T cells. To determine whether CD4 + CD57 + T cells were also expanded in human tuberculosis, we analysed (by flow cytometry) the phenotype of peripheral blood CD4 + T cells from 30 tuberculosis patients and 30 healthy controls. We observed a significant increase in the CD4 + CD57 + T‐cell subset in tuberculosis patients in comparison to healthy controls ( P < 0·001). Most CD4 + CD57 + T cells exhibited a CD28 − CD45RO + CD62L − phenotype, which is associated with memory cells. In vitro , a higher number of antigen‐stimulated CD4 + CD57 + T cells produced intracellular interferon‐γ and interleukin‐4 compared with antigen‐stimulated CD4 + CD57 − T cells ( P < 0·001). These findings suggest that the majority of CD4 + CD57 + T cells correspond to a phenotype of activated memory T cells.
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