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Expression of thePer1 gene in the hamster: Brain atlas and circadian characteristics in the suprachiasmatic nucleus

2001; Wiley; Volume: 430; Issue: 4 Linguagem: Inglês

10.1002/1096-9861(20010219)430

1096-9861

Shuzo Yamamoto, Yasufumi Shigeyoshi, Yoshiki Ishida, Tsuyoshi Fukuyama, Shun Yamaguchi, Kazuhiro Yagita, Takahiro Moriya, Shigenobu Shibata, Naoyuki Takashima, Hitoshi Okamura,

Psychological and Temporal Perspectives Research

Journal of Comparative NeurologyVolume 430, Issue 4 p. 518-532 Article Expression of the Per1 gene in the hamster: Brain atlas and circadian characteristics in the suprachiasmatic nucleus Shuzo Yamamoto, Shuzo Yamamoto Department of Anatomy and Brain Science, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, Japan Department of Orthopedics, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, JapanSearch for more papers by this authorYasufumi Shigeyoshi, Yasufumi Shigeyoshi Department of Anatomy and Brain Science, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, Japan Department of Anatomy, Kinki University School of Medicine, Osaka-Sayama, Osaka 589-8511, JapanSearch for more papers by this authorYoshiki Ishida, Yoshiki Ishida Department of Anatomy and Brain Science, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, JapanSearch for more papers by this authorTsuyoshi Fukuyama, Tsuyoshi Fukuyama Department of Anatomy and Brain Science, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, JapanSearch for more papers by this authorShun Yamaguchi, Shun Yamaguchi Department of Anatomy and Brain Science, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, JapanSearch for more papers by this authorKazuhiro Yagita, Kazuhiro Yagita Department of Anatomy and Brain Science, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, JapanSearch for more papers by this authorTakahiro Moriya, Takahiro Moriya Department of Pharmacology and Brain Science, School of Human Sciences, Waseda University, Tokorozawa 359-1192, JapanSearch for more papers by this authorShigenobu Shibata, Shigenobu Shibata Department of Pharmacology and Brain Science, School of Human Sciences, Waseda University, Tokorozawa 359-1192, JapanSearch for more papers by this authorNaoyuki Takashima, Naoyuki Takashima Department of Anatomy and Brain Science, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, JapanSearch for more papers by this authorHitoshi Okamura, Corresponding Author Hitoshi Okamura okamurah@kobe-u.ac.jp Department of Anatomy and Brain Science, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, JapanDepartment of Anatomy and Brain Science, Kobe University School of Medicine, Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanSearch for more papers by this author Shuzo Yamamoto, Shuzo Yamamoto Department of Anatomy and Brain Science, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, Japan Department of Orthopedics, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, JapanSearch for more papers by this authorYasufumi Shigeyoshi, Yasufumi Shigeyoshi Department of Anatomy and Brain Science, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, Japan Department of Anatomy, Kinki University School of Medicine, Osaka-Sayama, Osaka 589-8511, JapanSearch for more papers by this authorYoshiki Ishida, Yoshiki Ishida Department of Anatomy and Brain Science, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, JapanSearch for more papers by this authorTsuyoshi Fukuyama, Tsuyoshi Fukuyama Department of Anatomy and Brain Science, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, JapanSearch for more papers by this authorShun Yamaguchi, Shun Yamaguchi Department of Anatomy and Brain Science, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, JapanSearch for more papers by this authorKazuhiro Yagita, Kazuhiro Yagita Department of Anatomy and Brain Science, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, JapanSearch for more papers by this authorTakahiro Moriya, Takahiro Moriya Department of Pharmacology and Brain Science, School of Human Sciences, Waseda University, Tokorozawa 359-1192, JapanSearch for more papers by this authorShigenobu Shibata, Shigenobu Shibata Department of Pharmacology and Brain Science, School of Human Sciences, Waseda University, Tokorozawa 359-1192, JapanSearch for more papers by this authorNaoyuki Takashima, Naoyuki Takashima Department of Anatomy and Brain Science, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, JapanSearch for more papers by this authorHitoshi Okamura, Corresponding Author Hitoshi Okamura okamurah@kobe-u.ac.jp Department of Anatomy and Brain Science, Kobe University School of Medicine, Chuo-ku, Kobe 650-0017, JapanDepartment of Anatomy and Brain Science, Kobe University School of Medicine, Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanSearch for more papers by this author First published: 18 January 2001 https://doi.org/10.1002/1096-9861(20010219)430:4 3.0.CO;2-HCitations: 56Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Recent progress in study on the molecular component of mammalian clocks has claimed that mammals and Drosophila share the similar fundamental clock oscillating system. In the present study, we investigated expression of Per1, the first gene of the mammalian homolog of the Drosophila clock gene period, in the hamster brain, and we also examined its circadian expression pattern in the mammalian clock center, the suprachiasmatic nucleus (SCN). In situ hybridization using isotope-labeled cRNA probes revealed a wide and region-specific distribution of Per1 in the hamster brain and spinal cord. High levels of Per1 were found in the internal granular layer of the granular cells of the olfactory bulb, anterior olfactory nuclei, tenia tecta, olfactory tubercle, piriform cortex, suprachiasmatic nucleus, and gyrus dentatus of hippocampus. Moderate levels of expression were detected in many brain regions including the granular layer of the cerebellum, anterior paraventricular thalamic nucleus, caudate-putamen, inferior colliculus, pontine nuclei, inferior olive, and nucleus of the solitary tract. We examined the circadian profile of hamster Per1 mRNA in the SCN in constant darkness and found that Per1 expression showed a peak at subjective day (circadian time [CT] 4) and formed a trough at subjective night (CT16–CT20). A brief exposure of light at CT16 could acutely induce large quantities of Per1 mRNA in the hamster SCN, except for its dorsomedial subdivision. These findings suggest that the characteristics of Per1 gene expression in the mammalian circadian center (showing a peak in the daytime and a trough in the nighttime and a rapid inducibility by light) are common among mammalian species. Lastly, in hamster brain, Per1 gene is also inducible in extra-SCN brain nuclei, since light at night also elicited Per1 mRNA in neurons of the hypothalamic paraventricular nucleus. J. Comp. Neurol. 430:518–532, 2001. © 2001 Wiley-Liss, Inc. Citing Literature Volume430, Issue419 February 2001Pages 518-532 RelatedInformation