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Polimorfismo genético de la apolipoproteína E en un grupo de escolares del centro-oriente colombiano: comparación con las concentraciones plasmáticas de lípidos y apolipoproteínas

2007; National Institute of Health; Volume: 27; Issue: 4 Linguagem: Inglês

10.7705/biomedica.v27i4.171

2590-7379

Ney Callas, Elpidia Poveda, César Baracaldo, Patricia Hernández-Rodríguez, Carlina Castillo, Martha Guerra,

Cancer, Lipids, and Metabolism

Introduction. Research in laboratories around the world has documented the contribution of the E apolipoprotein alleles to structural variations of lipids and apolipoproteins.Objective. The gene frequencies of the E apolipoprotein alleles were compared with the lipid and apolipoprotein levels in school age children.Materials and methods. Six hundred and ninety one 5 to 15 years old school age children from the Colombian departments of Cundinamarca, Boyacá, Meta, Santander and Norte de Santander, were evaluated.The genotypes of the E apolipoprotein were identified by polymerase chain reaction-restriction fragment length polymorphism. Plasma levels for the following 5 lipids and lipoproteins were assayed: total cholesterol, HDL (high density lipoprotein) cholesterol, LDL (low density lipoprotein) cholesterol, triglycerides, VLDL (very low density lipoprotein) cholesterol, A-I apolipoprotein and B-100 apolipoprotein.Results. Alleles e2, e3 and e4 were found in frequencies of 0.04, 0.86 and 0.08, respectively. The E4 group (E4/3-E4/4), in contrast with the E2 group (E3/2-E2/2), presented highest plasma concentrations of total cholesterol, LDL cholesterol and B-100 apolipoprotein (p=0.014, 0.001 and 0.000, respectively). When the E3/3 group was compared with E2, the same result was obtained (p=0.015, 0.002 and 0.002, respectively). The influence of the E apolipoprotein polymorphism appeared greater in female children than male.Conclusions. The e4 allele was associated with higher levels of total cholesterol, LDL cholesterol and B-100 apolipoprotein and indicates the necessity of additional research into the interactionsbetween polymorphism E apolipoprotein and other genes, life styles, risk factors and potential contribution to cardiovascular diseases.