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Artigo Acesso aberto Produção Nacional Revisado por pares
BIBTEX RIS

Formulations of essential oils obtained from plants traditionally used as condiments or traditional medicine active against Staphylococcus aureus isolated from dairy cows with mastitis

2023; Oxford University Press; Volume: 76; Issue: 3 Linguagem: Inglês

10.1093/lambio/ovad034

ISSN

1472-765X

Autores

Kamylla Rafaella Sena Albuquerque, Gislaine Aparecida Purgato, Mayra Soares Píccolo, Fábio Assad Féres Rodrigues, Virgínia Ramos Pizziolo, Gaspar Diaz‐Muñoz, Ciro César Rossi, Marisa Alves Nogueira Diaz,

Tópico(s)

Transgenic Plants and Applications

Resumo

The prophylactic and therapeutic overuse of antimicrobials on the farm has contributed to the emergence of hard-to-fight bacterial strains causing bovine mastitis. Aiming at alternative therapies, this study evaluated the antimicrobial activity of 20 essential oils against clinical Staphylococcus aureus strains. Of them, five with strong activities were selected and evaluated for their minimum inhibitory concentrations (MIC) in culture medium and milk, cytotoxicity against bovine mammary cells (MAC-T), antiadhesive properties, and interactions among themselves and with cefoperazone. The oils remained active on milk, were not cytotoxic, and some concentrations stimulated MAC-T cells growth, suggesting healing potential. Subinhibitory concentrations of Coriandrum sativum, Origanum vulgare, Syzygium aromaticum, and Thymus vulgaris reduced biofilm formation by at least 80%. Several oil and cefoperazone combinations displayed additive interaction, with O. vulgare and C. sativum showing the most promising results. We developed formulations for being used as prophylactic postdipping solutions in the field, containing different concentrations (1% or 3%) of the active oils, alone or in combination, with 3% glycerin, 1% Tween 80, and water. The formulations showed strong antimicrobial activity in milk and enhanced antiadhesive properties, specially when two oils were combined in the formula, indicating promising biotechnological and therapeutical applications.

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